Protein Info for MPMX19_06143 in Azospirillum sp. SherDot2

Annotation: Endonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 transmembrane" amino acids 135 to 151 (17 residues), see Phobius details PF00730: HhH-GPD" amino acids 48 to 182 (135 residues), 70.7 bits, see alignment E=6.4e-24

Best Hits

Swiss-Prot: 42% identical to END3_THEMA: Endonuclease III (nth) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K10773, endonuclease III [EC: 4.2.99.18] (inferred from 57% identity to sti:Sthe_1233)

Predicted SEED Role

"Endonuclease III (EC 4.2.99.18)" in subsystem Control of cell elongation - division cycle in Bacilli or DNA Repair Base Excision (EC 4.2.99.18)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (226 amino acids)

>MPMX19_06143 Endonuclease III (Azospirillum sp. SherDot2)
MSVQPATSPDFDIDEVFRRLRVAVAPYPKAAMFELRDRGYATPFQQLVASLISARTRDET
SLAVAERLFAVAGTPAAMAALPEERLVELLHGATFPEPKARDIRALSRRIVEELGGEVPD
TPDGLMRFHGVGPKIAALTLAVGFGIPALAVDIHVHRIVNRWGYVQARTPEKTMAALLAV
LPKRYWVEINERLVPFGKWVCTGERPRCSTCPLLPMCARVGVTTSR