Protein Info for MPMX19_06094 in Azospirillum sp. SherDot2

Annotation: Lactate utilization protein B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00273: iron-sulfur cluster-binding protein
  • amino acids 17 to 455 (439 residues), 474.6 bits, E=1.5e-146
• PF02589: LUD_dom
  • amino acids 69 to 293 (225 residues), 173.1 bits, E=2.7e-54
• PF13237: Fer4_10
  • amino acids 307 to 373 (67 residues), 36.8 bits, E=1.5e-12
• PF00037: Fer4
  • amino acids 308 to 324 (17 residues), 23.3 bits, see alignment (E = 2.2e-08)
• PF13183: Fer4_8
  • amino acids 310 to 376 (67 residues), 52.5 bits, E=2.8e-17
• PF12800: Fer4_4
  • amino acids 311 to 325 (15 residues), 16.1 bits, see alignment (E = 5.8e-06)
  • amino acids 359 to 374 (16 residues), 14.2 bits, see alignment (E = 2.4e-05)
• PF13534: Fer4_17
  • amino acids 311 to 376 (66 residues), 30 bits, E=3.2e-10
• PF11870: LutB_C
  • amino acids 384 to 470 (87 residues), 75.2 bits, E=2.1e-24

Best Hits

Swiss-Prot: 42% identical to LUTB_BACVZ: Lactate utilization protein B (lutB) from Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42)

KEGG orthology group: None (inferred from 94% identity to azl:AZL_e03860)

MetaCyc: 43% identical to component of an iron-sulfur oxidase linked to L-lactate utilization (Bacillus subtilis subtilis 168)
L-lactate dehydrogenase. [EC: 1.1.1.27]

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF" in subsystem L-rhamnose utilization or Lactate utilization

MetaCyc Pathways

• homolactic fermentation (12/12 steps found)
• superpathway of glucose and xylose degradation (15/17 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• Bifidobacterium shunt (13/15 steps found)
• heterolactic fermentation (15/18 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to acetate and lactate II (4/4 steps found)
• pyruvate fermentation to (S)-lactate (1/1 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (9/13 steps found)
• L-histidine degradation V (1/2 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.27

Use Curated BLAST to search for 1.1.1.27

Protein Sequence (474 amino acids)

>MPMX19_06094 Lactate utilization protein B (Azospirillum sp. SherDot2)
MDGNAPDFAAASHAALADPQLRGNFRRAMDGLMSKRAAQFADAGEWQGVRALAASVRLRA
LSKLPELLETLEENCTRNGITVHWAATTDEANAIALDILHRVDAKLVVKGKSMVTEEMHL
NAVLEKDGIEVLESDLGEYIVQLDGTMPSHIIMPAIHLNTKQIAHLFKRKIKDAESREDA
AYLTDLARRVLRAKFQAADVGMSGVNAAVAETGTLCLIENEGNGRMCTTVPPVHIAFMGL
EKVVEKLEDVPPVISLLPRSATGQPITTYVNMISGPRKPGEKDGPREVHLVILDNGRSSI
YADPELRDTLRCIRCGACMNHCPVYTKVGGHAYEAPYPGPIGKILVPQIEGLAKRGAMPH
ACTMCNACVEICPVKIPIVEIMGRLRVEAVKPSGAVKDGGAKASRTEAMVWSGWAAMNAS
PTAYRVATLAMSKLGNAAPSSLPMLKEWTNVRTKPRFAGRTLHDLARAKGVPDV