Protein Info for MPMX19_05928 in Azospirillum sp. SherDot2

Annotation: Nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 901 PF04879: Molybdop_Fe4S4" amino acids 18 to 67 (50 residues), 58 bits, see alignment 1.5e-19 PF00384: Molybdopterin" amino acids 74 to 495 (422 residues), 236.6 bits, see alignment E=9e-74 PF01568: Molydop_binding" amino acids 594 to 709 (116 residues), 83.9 bits, see alignment E=1.7e-27 PF04324: Fer2_BFD" amino acids 844 to 893 (50 residues), 49.7 bits, see alignment 7.5e-17

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 90% identity to azl:AZL_e03480)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (901 amino acids)

>MPMX19_05928 Nitrate reductase (Azospirillum sp. SherDot2)
MFDGPDPSGGPSSGPKREVKTTCPYCGVGCGVIATVAATGAVSVRGDADHLANRGRLCSK
GSALADTLVPDGRLLHPEVDGQRVSWDEALDEVARRFSDTIARHGPDSVAFYVSGQLLTE
DYYVANKLMKGFIGSANIDTNSRLCMASSVVGHKRGLGADAVPGSYEDFECADLVVLVGS
NLAWCHPVLHQRLLAARAERGTRIVVVDPRRTDCVDAADRHLALKAGTDALLFNGLLAYL
DSNGHRDAGFVAAHAEGVEEALAAARADAPDIATVARRCGLEAAEVAAFYAEFAATDRVV
TVYSQGVNQSSQGSDTVNAILNLHFLTGRIGHPGCGPFSVTGQPNAMGGREVGGLANQLA
AHMGFTATEVDRVGRFWNAPRVAAKPGLKAVDLFHAIEAGTVKAVWIMATNPAVSMPDAG
RVQRALERCETVVVSDCIRDTDTGRFAHIRLPAKGWSEKDGTVTNSDRTISRQRAFKVEA
GEAQADWWIVTQVARRMGFADAFPYESAADLFREHAALSAFENGGTRAFDIGALGGLDDA
GYDALAPFPWPWRTGGEPQKRLFGDGRFFTADGRARMLPVTAKPLPRSVPDGALLLNTGR
LRDQWHTMTRTGLSARLSAHAPEPRLDVNPADALARGLVDGGLARITTVVGEAAGEALAR
VRVTDAQGEGTVFLPMHWTDRFTGGCVVGRLVDDAAVDPLSGQPDLKRMPATVAPWRPDW
TAFLLVRHAVEPGHGGYWARRAVAGGHLVEMAGTGAIPAVESWLPGLDPAEAVVEFRDEA
RGGLRMAWMKGDRLEACLFVAADGRLPPRGWLAGLLETDSLEDGARAGLLAGRAPGVPAD
EGRIVCACFSVGINRLVGAIRDRRLTSVAEIGAALKAGTNCGSCVPELKEVLRHAHQREV
A