Protein Info for MPMX19_05873 in Azospirillum sp. SherDot2

Annotation: D-alanyl-D-alanine carboxypeptidase DacA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 transmembrane" amino acids 42 to 63 (22 residues), see Phobius details PF00768: Peptidase_S11" amino acids 64 to 287 (224 residues), 236.1 bits, see alignment E=6e-74 PF13354: Beta-lactamase2" amino acids 74 to 223 (150 residues), 47.1 bits, see alignment E=2.9e-16 PF07943: PBP5_C" amino acids 310 to 399 (90 residues), 64.7 bits, see alignment E=1e-21

Best Hits

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 91% identity to azl:AZL_e00040)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>MPMX19_05873 D-alanyl-D-alanine carboxypeptidase DacA (Azospirillum sp. SherDot2)
MVHGNGLRKTHPSPQGIHRVIVVNPRPRTEGRSAALLCRLSIRHLAGCLSLAAAMLLSAP
ALALSQPDTAAKQAYLVDLSSGDVLLDKNGTVRMAPSSMTKMMTAYLTYDALVHGRTTLD
TSFPVSQTAWKMGGSRMFLKLGSAVRVEDLLRGLLIDSGNDAAVALAEGLAGSESAFAGM
MNAKARELGMADSHFMNASGWPDPGHYTTARDLATLATHLIRDFPQFYHYESERNFAWNG
IRQGNRNPLLYHPVSVDGIKTGHTEVGGYGLTASGERNGRRLVLVVNGLPSPQARNDEPT
RLLDWAWNSFKLYPLLRKGELVEQAPVWMGEEDSVPVTVADDVTVTLAPADRNSLHVVAT
LAEPLPSPVHRGDVVGRLRIDYDGAVRRQVDLVAGADVPASSGLTALGQRLGHLLP