Protein Info for MPMX19_05862 in Azospirillum sp. SherDot2

Annotation: 3-carboxy-cis,cis-muconate cycloisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02426: 3-carboxy-cis,cis-muconate cycloisomerase
  • amino acids 9 to 351 (343 residues), 434 bits, E=1.7e-134
• PF00206: Lyase_1
  • amino acids 16 to 300 (285 residues), 188.3 bits, E=2.3e-59
• PF10397: ADSL_C
  • amino acids 367 to 444 (78 residues), 65.5 bits, E=4.5e-22

Best Hits

Swiss-Prot: 60% identical to PCAB_PSEAE: 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 90% identity to azl:AZL_d00480)

MetaCyc: 47% identical to nitrosuccinate lyase (Streptomyces cremeus)
RXN-22527 [EC: 4.3.99.5]

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• aromatic compounds degradation via β-ketoadipate (7/9 steps found)
• nitrite biosynthesis (1/2 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aerobic toluene degradation (11/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.99.5 or 5.5.1.2

Protein Sequence (456 amino acids)

>MPMX19_05862 3-carboxy-cis,cis-muconate cycloisomerase (Azospirillum sp. SherDot2)
MTTIHADPLLDPLFTSDAAADAFSPSARLQGMLDFEAALARAEAAVGVIPARAVAAIEAA
CRAGLYDLTALGAEAALAGNTAIPLVKHLTATVKAADEEASRYVHWGATSQDAMDSGLML
QLRRFLDGLDADLAALADGLAGLAEHHRTTPMVARTWLQHALPTTFGLKAAGWLDALGRD
RQRIAATRTRLALQFGGAAGTLAALGDSGPAVAEAVAKELDLPLPALPWHASRDRIAELA
SALGILAGTLGTLGRDISLMMQMEVGEAFEPSAPGRGGSSTMPHKRNPVSCAVLLSTAIR
APALVGGLLAAQVQEHERGLGGWHAEWQALPDLCRLVAGAARHARETIAGLTVDAERMRA
NLDLTRGLILAEAVTMALGDRMGRMAAHSRVAEASRRAAADARPLRDVLAEDGEVVQALG
ADGLDRLFDPLRYTGAASHFIDRVLDSHRAQQRQQN