Protein Info for MPMX19_05723 in Azospirillum sp. SherDot2

Annotation: Carbamoyl dehydratase HypE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 TIGR02124: hydrogenase expression/formation protein HypE" amino acids 18 to 348 (331 residues), 465.8 bits, see alignment E=3.4e-144 PF00586: AIRS" amino acids 49 to 162 (114 residues), 83 bits, see alignment E=2.1e-27 PF02769: AIRS_C" amino acids 174 to 325 (152 residues), 97.2 bits, see alignment E=1.1e-31

Best Hits

Swiss-Prot: 72% identical to HUPE_AZOCH: Hydrogenase expression/formation protein HupE (hupE) from Azotobacter chroococcum mcd 1

KEGG orthology group: K04655, hydrogenase expression/formation protein HypE (inferred from 95% identity to azl:AZL_d02020)

MetaCyc: 49% identical to carbamoyl dehydratase HypE (Escherichia coli K-12 substr. MG1655)
4.2.1.M8 [EC: 4.2.1.M8]; RXN-22648 [EC: 4.2.1.M8]

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypE" in subsystem NiFe hydrogenase maturation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.M8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (348 amino acids)

>MPMX19_05723 Carbamoyl dehydratase HypE (Azospirillum sp. SherDot2)
MTDRRLFTRKLDIARGAVDMTHGSGGKAMAQLVRELFAAAFANPWLDQGNDQAAFEVPGG
RMVMTTDGFVVSPLFFPGGDIGSLAVHGTVNDVAMAGAVPLHLTAGFIIEEGFPLADLKR
VVDSMAAAAREAGVAIVSGDTKVVERGKGDGIFITTTGIGMVPPGVAPSGDRARPGDVIL
VSGTMGDHGVAIMSTRDNLGFETAILSDSAALNGLVAAMVAAVPDVHVLRDPTRGGLATT
LNEIAHQSGVGMRLRESDIPVRAQVTAACELLGLDPLYAANEGKLIAICTGEECDRLLSV
MRDHPLGRDAACIGRVVEDPHRFVQMETRFGGRRIVDWLTGEQLPRIC