Protein Info for MPMX19_05700 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 TIGR01849: polyhydroxyalkanoate depolymerase, intracellular" amino acids 2 to 404 (403 residues), 604.7 bits, see alignment E=3.7e-186 PF06850: PHB_depo_C" amino acids 204 to 405 (202 residues), 307.8 bits, see alignment E=1.5e-96

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 96% identity to azl:AZL_d02230)

Predicted SEED Role

"Polyhydroxyalkanoic acid synthase" in subsystem Polyhydroxybutyrate metabolism

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>MPMX19_05700 hypothetical protein (Azospirillum sp. SherDot2)
MLYHLYDMQHAAMRPMRFWAEAAQQTFQNPLMPLSYTKLGRAVAAGAELVERTTRRFGKP
AFGLPETVVDGQTVPVTERFVWQSPFCQLLNFAKPEGTPRQPKVLLVAPMSGHHATLLRG
TVEALMRDHDVYITDWIDARLVPMSAGRFDLDDYIDTVAELIRFLGPNTHVIAVCQPAVP
VMAAVSLMAAADEPVQARSMTLMGGPIDPMANPTVPVKLAMERPLKWFERSVITTVPVYY
PGAFRRVYPGFIQLSGFMSMNLDRHINEHVGLFRHLVRGDGDSAEQHRRFYDEYLSVMDL
SADFYLQTIETVFQKHALPEGTMVSRGRKVEPSAIAKTAVMTVEGELDDISAPGQTIAAQ
RLCSSLPDDMRKTHFQKGVGHYGIFNGRRWRESILPEIRSFIQSHDGE