Protein Info for MPMX19_05650 in Azospirillum sp. SherDot2

Annotation: Ribonuclease HII

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 PF01351: RNase_HII" amino acids 31 to 212 (182 residues), 138.3 bits, see alignment E=1.5e-44

Best Hits

Swiss-Prot: 54% identical to RNH2_RHORT: Ribonuclease HII (rnhB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K03470, ribonuclease HII [EC: 3.1.26.4] (inferred from 93% identity to azl:AZL_d02320)

MetaCyc: 51% identical to RNase HII (Escherichia coli K-12 substr. MG1655)
Ribonuclease H. [EC: 3.1.26.4]

Predicted SEED Role

"Ribonuclease HII (EC 3.1.26.4)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA replication, archaeal or Ribonuclease H (EC 3.1.26.4)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.4

Use Curated BLAST to search for 3.1.26.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (221 amino acids)

>MPMX19_05650 Ribonuclease HII (Azospirillum sp. SherDot2)
MPAKAPRKAAIPRQRPDLSLEAGFGPGRRVCGIDEVGRGPLCGPVVTAAVVLPPEGLPPE
IAALIDDSKAVNRRLREEIEPAIRAHALAFAIAEASAAEIDELNIHKATLLAMKRAYLAL
PGPAPDAALIDGKFTPDIACERVAVVKGDSRSQSIAAASILAKVARDSEMLRLSVQYPHY
GWDRNAGYPTPEHLEALTRHGVTPHHRVSFAPVKRQRALSG