Protein Info for MPMX19_05331 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 846 PF00359: PTS_EIIA_2" amino acids 11 to 146 (136 residues), 99.1 bits, see alignment E=6e-32 PF00381: PTS-HPr" amino acids 166 to 243 (78 residues), 67.3 bits, see alignment E=2.5e-22 TIGR01003: phosphocarrier, HPr family" amino acids 166 to 243 (78 residues), 51.2 bits, see alignment E=9.4e-18 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 284 to 841 (558 residues), 465.3 bits, see alignment E=2.7e-143 PF05524: PEP-utilisers_N" amino acids 286 to 404 (119 residues), 61.3 bits, see alignment E=2.6e-20 PF00391: PEP-utilizers" amino acids 434 to 505 (72 residues), 73.5 bits, see alignment E=2.1e-24 PF02896: PEP-utilizers_C" amino acids 531 to 817 (287 residues), 364.7 bits, see alignment E=8.4e-113

Best Hits

Swiss-Prot: 66% identical to PTFAX_XANCP: Multiphosphoryl transfer protein (fruB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 91% identity to azl:AZL_c02630)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (846 amino acids)

>MPMX19_05331 hypothetical protein (Azospirillum sp. SherDot2)
MLTDTHPLSLPPDLIRLGAAPAGKEAAIREAAQLLIAAGCIDPAYADSMIRRETVANTFL
GHGVAIPHGMVDDRNLVRHNGIAILQVPDGVVWNDGQTARLVVAIAAQSDAHIAILRRLT
RLMQDEARLAALFTTGDPADLVAALSDETASPVPSAADAGDLAENFEWVVDYPTGLHARP
AAVWVESARAASGRVRVRHGEESADGKTLVALLQLGLRPGDRITVSTDGPDARAVLNRLR
ATITGLSAREKADAAAAARKAKAVVQGWTPPATGTNGGREMPAIAGIGASPGLTIGPVHV
MPKADLTVPDRPAPLLEGGNRLHEALNLTRQQLKALADDTARRLGPAEAGIFAAQAELLS
DTDVITLACQLMVEGHGPGWSWNEAVERSAARLAANPNPVLAGRAADLRDVGRRVLTRID
PDLRGGSIRDLPDTPCILVADDLSPSDTAALDMGRVIGLATAQGGPTSHTAILARTLGLP
AMVAGGAALTGLANGTTAILDGQSGRLYLDPSEADIAAAHGWIEEQRIRKTQQEERRSLP
ARTRDGHTVEIGANVNRPDQVAMALSQGGEGVGLMRTEFLFLERGDAPSEEEQYETYRAM
LEALDGRPLIVRALDIGGDKQVPHLQLPHEENPFLGVRGARLLLRHPELLEPQLRALYRA
AKGAKPGALLIMFPMITTLREVETLRAVCDRIRAELDAPAVPLGIMVEVPAAAIQADVLA
RHVDFFSIGTNDLTQYALAIDRQHPELAAEADSLHPSVLRLIRLTVEGAEKHGRWVGVCG
GIAGDPFGAALLAGLGVRELSMTPRDIPGVKDKLRDSDLVGLRALAQRALDCESSDEVRA
LEGEAS