Protein Info for MPMX19_05329 in Azospirillum sp. SherDot2

Annotation: PTS system fructose-specific EIIB'BC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 250 to 274 (25 residues), see Phobius details amino acids 285 to 308 (24 residues), see Phobius details amino acids 323 to 346 (24 residues), see Phobius details amino acids 366 to 386 (21 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details amino acids 433 to 459 (27 residues), see Phobius details amino acids 480 to 499 (20 residues), see Phobius details amino acids 506 to 526 (21 residues), see Phobius details amino acids 546 to 567 (22 residues), see Phobius details PF02302: PTS_IIB" amino acids 14 to 96 (83 residues), 24.5 bits, see alignment E=1.7e-09 amino acids 115 to 204 (90 residues), 71.5 bits, see alignment E=3.9e-24 TIGR00829: PTS system, Fru family, IIB component" amino acids 114 to 197 (84 residues), 114.8 bits, see alignment E=1.8e-37 TIGR01427: PTS system, Fru family, IIC component" amino acids 229 to 569 (341 residues), 466.5 bits, see alignment E=6.2e-144

Best Hits

Swiss-Prot: 68% identical to PTFBC_XANCP: PTS system fructose-specific EIIB'BC component (fruA) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 90% identity to azl:AZL_c02610)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (590 amino acids)

>MPMX19_05329 PTS system fructose-specific EIIB'BC component (Azospirillum sp. SherDot2)
MATLLAVIAAGDLTTQAVLAAEALRKAAAKAGHRIQVEIRTSLGIQSPLDAAALSTADGL
ILIGSGDFDDGRFAALKRSTASLDEVLGNPAAVLEKASSSASAASAAAGPRRIVAITSCP
TGIAHTFMAAEGIQQAATALGHKVRVETQGSVGARDTLTDEEIREADVVLIAADTQIDLS
RFAGKRVFLSGTKPAINDGKALVNRALAEAKTHGAAGGATPLADAVAAGKAERSAQRTGP
YKHLMTGVSFMLPFVVTGGLLIALAFALGGIYVYEESNAGTLGYALFQIGAKGAFALMVP
ALAGYIAYSIADRPGIAPGMVGGMLAGSIGAGFLGGIVAGFIAGYGTAFLNRHIKLHRNL
EGLKPVLILPLLGSLLTGLLMIYVVGTPVAEALAWMSAWLKGMQGSSAILLGLLIGAMMA
FDMGGPVNKAAYAFSTGLIASQVYTPMAAAMAAGMVPPLGLALATKLFADRFTAEEREAG
NAAAVLGIAFITEGAIPFAARDPLRVIPALMLGGALTGAMSMAVGAELKVPHGGVFVLPI
PNAVTHLLGYVVALVVGTVVTAVALRILKKPVAVTAAAPKPAVRQAGAHA