Protein Info for MPMX19_05286 in Azospirillum sp. SherDot2

Annotation: putative Ni/Fe-hydrogenase B-type cytochrome subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 transmembrane" amino acids 39 to 59 (21 residues), see Phobius details amino acids 79 to 99 (21 residues), see Phobius details amino acids 119 to 134 (16 residues), see Phobius details amino acids 147 to 171 (25 residues), see Phobius details amino acids 201 to 221 (21 residues), see Phobius details TIGR02125: Ni/Fe-hydrogenase, b-type cytochrome subunit" amino acids 31 to 243 (213 residues), 250.5 bits, see alignment E=6e-79 PF01292: Ni_hydr_CYTB" amino acids 32 to 238 (207 residues), 115 bits, see alignment E=1.8e-37

Best Hits

Swiss-Prot: 67% identical to CYBH_CUPNH: Probable Ni/Fe-hydrogenase B-type cytochrome subunit (hoxZ) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K03620, Ni/Fe-hydrogenase 1 B-type cytochrome subunit (inferred from 89% identity to azl:AZL_c02010)

MetaCyc: 67% identical to hydrogenase b-type cytochrome subunit (Cupriavidus necator)
Hydrogenase (acceptor). [EC: 1.12.99.6]

Predicted SEED Role

"Ni,Fe-hydrogenase I cytochrome b subunit" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.99.6

Use Curated BLAST to search for 1.12.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (264 amino acids)

>MPMX19_05286 putative Ni/Fe-hydrogenase B-type cytochrome subunit (Azospirillum sp. SherDot2)
MAPLDDAKPVATPDEEGTAAPAAGRFLKAIYVYEVPVRIWHWVNALAIVVLSVTGYLIAN
PLPTMPGEASANFLMGYIRFVHFAAAYIFAIGFIGRLYWALVGNHHAKQLFRFPFWDRHW
WGELFLEARWYLFLERKPKLYVGHNPLAQFSMFWAIAVGSLFMIVTGFALYSEGAGQGSW
QDTLFGWVIPLFGQSMDVHTWHHLGMWAILLFVIVHVYAAVREDIMSRQSIISTMISGVR
TFKDAEAPADEEHERPVKKAARAG