Protein Info for MPMX19_04970 in Azospirillum sp. SherDot2

Annotation: Protein-glutamate methylesterase/protein-glutamine glutaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 994 PF01339: CheB_methylest" amino acids 28 to 203 (176 residues), 182.4 bits, see alignment E=2.3e-57 PF03705: CheR_N" amino acids 231 to 279 (49 residues), 51.7 bits, see alignment 2e-17 PF01739: CheR" amino acids 294 to 484 (191 residues), 181 bits, see alignment E=6.3e-57 PF13596: PAS_10" amino acids 738 to 843 (106 residues), 91.6 bits, see alignment E=1.6e-29 PF08448: PAS_4" amino acids 744 to 846 (103 residues), 34 bits, see alignment E=1.1e-11 amino acids 856 to 973 (118 residues), 41.1 bits, see alignment E=6.7e-14 PF13188: PAS_8" amino acids 850 to 884 (35 residues), 19.4 bits, see alignment (E = 2.8e-07)

Best Hits

KEGG orthology group: K13924, two-component system, chemotaxis family, CheB/CheR fusion protein [EC: 2.1.1.80 3.1.1.61] (inferred from 91% identity to azl:AZL_c05240)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80, 3.1.1.61

Use Curated BLAST to search for 2.1.1.80 or 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (994 amino acids)

>MPMX19_04970 Protein-glutamate methylesterase/protein-glutamine glutaminase (Azospirillum sp. SherDot2)
MPATSPNADAAGVPAESGAPPEQACCIVGIGASAGGLEALQRFFDAVPGDSDLAYVVVQH
LSPVHKSLMVDLLAKHTAMGVVQATEGVPVQRNTVYLLPPGKHLSIEDSRLRLTAKDAAG
GMSLPIDIFLTALARDQGPFAIGLILSGTGSDGTRGLMAIKSAGGFTAVQQPETAQFDGM
PRSALATGQIDATLAPEDLPARLIEHARKALVRARPMPARQRREDGDDPLAQIIAVIRSV
TGVDFSHYKLATLLRRIERRMQGAGLDSMDEYAALLRRNSAETVNLYKEMLIGVTRFFRD
EAAFRVLADRVIPSLLANRSPTDMVRVWVCGCASGEEAYSLAILFAEAQERLGRSFDVKI
FATDIDQDSIEIASLGEYPGAIAEDVSQDRLSRFFVARGDSYAVSRDIRRMVVFAAHNIM
RDPPFTKIDLLSCRNLLIYMDQPLQRKVLSLFQYALRQSGFLFLGTSETLGDLQSDFHTV
DSRNKVFQSLRAGSFRLSRLLVPTVAAPLTRHGEDAVSHGMEEGAVIDEAIGALMKAYVP
PCLLVNEQMTIMHVFGDASSLLKVPAGEATLNVLKLLPSSVAMVTGTALNRAFRSGEEFA
LSNIPVKDREGVAAISLRVQPYVARKTGRRFALVVLDRANMLPLSVPDSDFDLNADAAER
IRGLEQELLSRGENLQATIEELETANEELQATNEELLASNEELQSTNEELQSVNEELYSV
NAEYQAKVEELTEITNDLDNLLRSTEIGTVFLDGDFVIRRFTPAAARFINIIPRDLGRSI
AHLSTNIDYPDFLADIRKVFDGQEAVERHVRVHGGRWVLTRILPYLTDKSQIGGVVVTFV
DVTETKQAQERLQKVLDSLPEHIAVTDRDGRIVMVNAAWRGFAQRNGAPATERCGVGTNY
LDVCLNPEAAQKDAIARAVHDGLRRILNGEADDFSIEYPCHSPDEQRWFLMHATRLNDHD
GGIVVSHINITNRKLMELQVDGSGKGDRGDGGGE