Protein Info for MPMX19_04833 in Azospirillum sp. SherDot2

Annotation: Cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 14 to 419 (406 residues), 569.3 bits, E=2.2e-175
• PF00266: Aminotran_5
  • amino acids 38 to 407 (370 residues), 472.7 bits, E=1.2e-145
• PF00155: Aminotran_1_2
  • amino acids 48 to 257 (210 residues), 43.9 bits, E=2.8e-15
• PF01041: DegT_DnrJ_EryC1
  • amino acids 78 to 218 (141 residues), 21.3 bits, E=2.1e-08

Best Hits

Swiss-Prot: 50% identical to SUFS_SERP5: Cysteine desulfurase (sufS) from Serratia proteamaculans (strain 568)

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 96% identity to azl:AZL_c04300)

MetaCyc: 49% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), SufS subfamily" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• superpathway of L-alanine biosynthesis (3/4 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• molybdopterin biosynthesis (3/6 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (2/5 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Protein Sequence (420 amino acids)

>MPMX19_04833 Cysteine desulfurase (Azospirillum sp. SherDot2)
MSDAILNQAYDVQQVREDFPILARSVHARKGKPGKPLVYLDSGASAQKPRQVIQAMTRFL
EEDYSNIHRGVHFLSQTATDQFEAVRAKVAHFLNAPNDRCIVFTRSSTEAINLVASSYGR
TFLNEGDEVILSIMEHHANIVPWQLLQAEKKIVIKVVPCDEDGVLDMDAYRGLLSDRTKL
VAITHCSNVLGTVTPAKTIARLAHERGVPVLFDGSQAVVHGTVDVQDIDADFYVFTAHKL
YGPTGLGVLYGKYDLLKKMPPYQGGGDMIESVSFEGTTFKAPPSRFEAGTPPITEVIGLG
AAIDYVEALGRDAIAAHEHDLLQYATQQLSQIGGLRIVGTAPGKGPIVSFALDGVHPHDV
GTIVDQYGVAVRVGRHCAEPLAERFGVGPTVRASFGLYNTREEADALVQSLQAVKEFFGA