Protein Info for MPMX19_04578 in Azospirillum sp. SherDot2

Annotation: dTDP-L-rhamnose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05368: NmrA
  • amino acids 2 to 91 (90 residues), 33 bits, E=1.8e-11
• PF01370: Epimerase
  • amino acids 3 to 127 (125 residues), 76.1 bits, E=1e-24
  • amino acids 170 to 277 (108 residues), 78.2 bits, E=2.4e-25
• PF02719: Polysacc_synt_2
  • amino acids 3 to 119 (117 residues), 26.2 bits, E=1.6e-09
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 126 (123 residues), 55.3 bits, E=2.8e-18
  • amino acids 167 to 339 (173 residues), 67.2 bits, E=6.6e-22
• PF01073: 3Beta_HSD
  • amino acids 5 to 126 (122 residues), 55.9 bits, E=1.2e-18
• PF13460: NAD_binding_10
  • amino acids 7 to 126 (120 residues), 47 bits, E=9.7e-16
• PF07993: NAD_binding_4
  • amino acids 64 to 258 (195 residues), 28.9 bits, E=2.3e-10

Best Hits

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 61% identity to sgr:SGR_6705)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• stachyose degradation (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• UDP-sugars interconversion (4/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (355 amino acids)

>MPMX19_04578 dTDP-L-rhamnose 4-epimerase (Azospirillum sp. SherDot2)
MRVLLTGGAGFIGGHVLAELLRRDHEVRVLDSLRPDVHPTPTAWAAPAGVEALIGDVRDA
AILDRAVAGVDTVIHLAAKVGLGVAIGDMPDYASSNEVGTAELLVAMARAGVSRLTLASS
MVVYGEGFGHCAEHGRVRPAPRLESALVQQRFEPPCPLCGQPLENLLVAEDTPFDPRNAY
ATSKAAQEFYAANWARSTGGTVAMMRYHNVYGPGMPRDTPYAGVASIFTSALRRGAAPRV
FEDGRQRRDFVHVRDVASATVTACEGQTAGVAAYNVGSGTPRTVGDMAEALSRALNGPPP
VVTGQYRLGDVRHITADSSRLCNRFDWRPRIGFAEGMAELAHLDPASPGTMVRLP