Protein Info for MPMX19_04484 in Azospirillum sp. SherDot2
Annotation: 2-iminobutanoate/2-iminopropanoate deaminase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 40% identical to Y322_HAEDU: RutC family protein HD_0322 (HD_0322) from Haemophilus ducreyi (strain 35000HP / ATCC 700724)
KEGG orthology group: None (inferred from 78% identity to bja:bll3976)MetaCyc: 31% identical to 2-iminobutanoate/2-iminopropanoate deaminase monomer (Salmonella enterica enterica serovar Typhimurium str. LT2)
Tryptophanase. [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2]; 3.5.99.10,4.3.1.-,4.3.1.19,4.4.1.1,4.4.1.1,4.4.1.2,3.5.99.7,4.4.1.11 [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2, 3.5.99.7, 4.3.1.19, 4.4.1.11, 4.4.1.2]
Predicted SEED Role
"RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein"
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-threonine metabolism (15/18 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
- L-threonine degradation I (6/6 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
- glycine betaine degradation III (6/7 steps found)
- D-serine degradation (3/3 steps found)
- L-cysteine degradation II (3/3 steps found)
- L-methionine degradation II (3/3 steps found)
- L-serine degradation (3/3 steps found)
- glycine degradation (3/3 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- glycine betaine degradation I (6/8 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- homocysteine and cysteine interconversion (3/4 steps found)
- L-tryptophan degradation II (via pyruvate) (2/3 steps found)
- seleno-amino acid detoxification and volatilization III (2/3 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- L-methionine biosynthesis II (4/6 steps found)
- L-cysteine biosynthesis III (from L-homocysteine) (1/2 steps found)
- L-threonine degradation V (1/2 steps found)
- dimethyl sulfide biosynthesis from methionine (1/2 steps found)
- seleno-amino acid detoxification and volatilization I (1/2 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- superpathway of L-cysteine biosynthesis (mammalian) (3/5 steps found)
- sulfolactate degradation III (1/3 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- seleno-amino acid biosynthesis (plants) (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
- superpathway of sulfolactate degradation (2/6 steps found)
- superpathway of L-methionine salvage and degradation (9/16 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
- glutathione-mediated detoxification II (1/9 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- superpathway of seleno-compound metabolism (7/19 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
- Cysteine metabolism
- Glycine, serine and threonine metabolism
- Methionine metabolism
- Nitrogen metabolism
- Propanoate metabolism
- Selenoamino acid metabolism
- Sulfur metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.17, 4.3.1.18, 4.3.1.19, 4.4.1.1, 4.4.1.11
Use Curated BLAST to search for 3.5.99.10 or 3.5.99.7 or 4.1.99.1 or 4.1.99.2 or 4.3.1.13 or 4.3.1.17 or 4.3.1.18 or 4.3.1.19 or 4.3.3.8 or 4.4.1.1 or 4.4.1.11 or 4.4.1.13 or 4.4.1.15 or 4.4.1.2 or 4.4.1.25 or 4.4.1.28 or 4.4.1.35 or 4.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (114 amino acids)
>MPMX19_04484 2-iminobutanoate/2-iminopropanoate deaminase (Azospirillum sp. SherDot2) MITRFQVGSRMSQAVVCQGMVHIAGQVANNRTGSIHEQTRDVLAKIDALLAEAGSDKSKL VAVNVFLPHITDFDAMNEVYDAWIDPANPPARACTEARLADPDLRVEMTALAAL