Protein Info for MPMX19_04471 in Azospirillum sp. SherDot2

Annotation: Bifunctional protein GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 7 to 132 (126 residues), 37.6 bits, E=5.4e-13
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 438 (432 residues), 491.2 bits, E=1.5e-151
• PF12804: NTP_transf_3
  • amino acids 8 to 138 (131 residues), 82.7 bits, E=8.8e-27
• PF00483: NTP_transferase
  • amino acids 9 to 212 (204 residues), 35 bits, E=3.1e-12
• PF00132: Hexapep
  • amino acids 267 to 301 (35 residues), 27.3 bits, 5.2e-10
  • amino acids 302 to 337 (36 residues), 30.6 bits, 4.9e-11
  • amino acids 380 to 413 (34 residues), 34.5 bits, 2.9e-12
• PF14602: Hexapep_2
  • amino acids 381 to 413 (33 residues), 30.1 bits, see alignment (E = 8.2e-11)

Best Hits

Swiss-Prot: 61% identical to GLMU_MAGSA: Bifunctional protein GlmU (glmU) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 97% identity to azl:AZL_a07260)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (450 amino acids)

>MPMX19_04471 Bifunctional protein GlmU (Azospirillum sp. SherDot2)
MAHRPLACVILAAGKGTRMKSDLPKVLHRVAGRPMVGHVLAAVKTLDPDHVVVVVGPGMD
SVAAAVAPYPTAVQHEQRGTADAVRAAFGLLEGFTGDVIVLYGDTPLVTPDTLRALVQAR
RQAADPAVVVLGMRPDDPGAYGRLILNARDGLEKIVEYLDASEEERAVTLCNAGLMAFDG
ARMFDLIGSVGNENAKSEYYLTDVVQIARRAGMACAVVEAAPAEVVGVNSRAELAEVEKL
LQRRLRKAAMDNGATLIDPDSTFLSVDTRLGRDVVVGPGCFFGAGVTVGDRVEIKPYCHL
EGVRIDSGAVIGPYARLRPGAEIGPDAHIGNFVEVKNAVIEAGAKANHLTYIGDARVGAK
ANIGAGTITCNYDGFSKSRTEIGAGAFIGSNSALVAPVVIGDGAIVGAGSVVTMAVEADA
LVVARGNQKAYAGWAKRFRERKQNEKAKKA