Protein Info for MPMX19_04343 in Azospirillum sp. SherDot2

Annotation: Efflux pump membrane transporter BepE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1042 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 332 to 352 (21 residues), see Phobius details amino acids 359 to 380 (22 residues), see Phobius details amino acids 386 to 410 (25 residues), see Phobius details amino acids 430 to 450 (21 residues), see Phobius details amino acids 462 to 481 (20 residues), see Phobius details amino acids 524 to 542 (19 residues), see Phobius details amino acids 593 to 612 (20 residues), see Phobius details amino acids 853 to 873 (21 residues), see Phobius details amino acids 879 to 899 (21 residues), see Phobius details amino acids 906 to 930 (25 residues), see Phobius details amino acids 950 to 971 (22 residues), see Phobius details amino acids 983 to 1008 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 4 to 1008 (1005 residues), 1031.6 bits, see alignment E=0 PF03176: MMPL" amino acids 299 to 485 (187 residues), 39.4 bits, see alignment E=3.7e-14 amino acids 804 to 1015 (212 residues), 25.2 bits, see alignment E=7.5e-10

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 96% identity to azl:AZL_a04330)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1042 amino acids)

>MPMX19_04343 Efflux pump membrane transporter BepE (Azospirillum sp. SherDot2)
MVLSDISIRRPVLATVMSLALMLIGIVSYQRLSVREYPKIDEPVVTVETTYKGASAQIIE
SQVTQTLEDSLAGIEGIDVMSSISRAEKSQITLRFRLDRNVDVAASDVRDRVGRVRAQLP
SEIDEPVIAKVEADAQPIIYLAFSSDRHSPLEVTDFADRYVKDRLQNLPGVAQVRIFGER
RFAMRLWLDPQRMAAYRVTPQDVENALRRQNVEIPAGRVESVAREFTVVSETDLRSPAEF
EAIILRDDSGYLVRLRDVGRAELGALDERVSARFNGRGAVAIGVVKQSTANPLDVSKAVN
DALPKIRAAVPDGMGVDVGYDSSVFIAKSIDAVFHTIFEAIILVVLVIFFFLRSLRATLV
PLVTIPVSLIGGFALMYALGFSINTLTLLSMVLAIGLVVDDAIVMLENIFRYVEEGMNPF
QAALKGSREIGFAVIAMTITLAAVYAPIGFMTGRTGRLFTEFALTLAGAVIVSGFVALTL
SPMMCSKLLKHETKHGLLYRAIERFLEGMTNGYRRLLRLSLRARPLVLLIGLGVAAASYF
LFTGLKSELSPVEDRGTIVGIAIAPEGSTLDYTMGYAQRMEALFRQIPVLEKFFVVVGFP
VVNQGIAFVRLIDWDEREVKQQAITAQLFPKMFGIPGILGFVTNPPSLGQSPIDKPVNFV
IQTSLPFEELQAMVNAMMAEARNFPGLTNLDTDLKLNKPELRVSLDRDKAADLGVDVDTV
GRTLETLLGGRQVTRFKKDGKQYDVIVQVANVDRRNPDDIAGIYVRGGTSATGGAGQMIS
LANLVKVEERVAPKELNHFNKLRSATITATLAPGTSLGEALAVMQAAANKVLPATAQTDY
AGQSREFRESATGLYFVFILALAFIYLVLAAQFESFVDPFVIMLTVPLSMTGALAALQMS
GGTMNVYSQIGLVTLVGLITKHGILIVEFANQLQRAGTDIRKAVEEAAVLRLRPILMTTG
AMVLGAVPLAYAKGAGAESRQAIGAVIVGGMTLGTLLTLFVVPTVYSYLARKKPMQDDTG
EAEQAASGHGGGQGHGAPHPAE