Protein Info for MPMX19_04252 in Azospirillum sp. SherDot2

Annotation: C4-dicarboxylate transport sensor protein DctB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 616 transmembrane" amino acids 39 to 59 (21 residues), see Phobius details amino acids 317 to 339 (23 residues), see Phobius details PF00512: HisKA" amino acids 386 to 452 (67 residues), 35.8 bits, see alignment E=7e-13 PF02518: HATPase_c" amino acids 498 to 605 (108 residues), 72.4 bits, see alignment E=4.1e-24

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 91% identity to azl:AZL_a05640)

Predicted SEED Role

"MISCELLANEOUS; Not classified regulator"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (616 amino acids)

>MPMX19_04252 C4-dicarboxylate transport sensor protein DctB (Azospirillum sp. SherDot2)
MLIHPGEQATDSTTPPAPAVTLSPAAQLVARALERPRRLLLAALLLGVPVAAGLAAGWAS
SLAQEDLRESARAQLALYNANLRAELERHAAVPLALAQDAEVRALLANPTPDAVDRLNRK
LQDIARALDALALYVMDTKGTTVAASNWNSPASFVGENFSYRPYFRMAMDQGEGHHFALG
TTSLVPGFYTANRVVAENRTVGAVVLKLGFDRLEAAWTPGQERLLVTDRDGVVFITNVPS
WRYHALPRRLPISLPIIPEGTSEGLDVLPWAFGGASDRIALEEKDGQKRYLLTSVAMPGG
DWTLHSLTSLDPVTAHAWVAGLLAAAAVAMAALAAYAVALRRVALVERIALQEESRAELE
RRVAAATADLRAAENELTQAAKLAALGQMSAGIAHEINQPLAAMRSFADNAVVLLERGRL
DSVRDNLAEIAELTDRMAAITRQLKGFARRASGTLGPVSVQGAVTQALALLEAKLRREDI
TVEADLPDQPVRVVAEDVRLQQVLVNLIGNAADAMRGCPVRRLRIGLVAAEGEALLSVAD
TGSGVAEADLPRLFVPFFTTKEAGEGLGLGLSISHGIVEDFGGSLTAANRNGKPGHGAVF
TLRLTTTEPSAKEQNQ