Protein Info for MPMX19_04123 in Azospirillum sp. SherDot2

Annotation: Asparagine synthetase [glutamine-hydrolyzing] 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03104: asparagine synthase family amidotransferase
  • amino acids 1 to 594 (594 residues), 1002.8 bits, E=4.1e-306
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 525 (524 residues), 437.3 bits, E=9.8e-135
• PF13522: GATase_6
  • amino acids 33 to 161 (129 residues), 132.6 bits, E=1.4e-42
• PF13537: GATase_7
  • amino acids 48 to 167 (120 residues), 142.1 bits, E=1.3e-45
• PF00733: Asn_synthase
  • amino acids 246 to 591 (346 residues), 323.5 bits, E=3.9e-100

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 71% identity to met:M446_2680)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (596 amino acids)

>MPMX19_04123 Asparagine synthetase [glutamine-hydrolyzing] 1 (Azospirillum sp. SherDot2)
MCGVSGEIRFKSGEAASTAAVQAMCDRLAPRGPDGHGLFAHGRVAFGHRRLKIIDLSEAS
QQPMVDSALGLAIVFNGCIYNHRELREELAGLGYSFFSDGDTEVLIKAYHAWGPDFVKRM
NGMFAFAIHERDSGRVVLGRDRLGIKPLYLADIPGGLRFASSLPALVASGGIDTDVDPVA
LHHYMSFHAVVPAPHTILKGVRKLPPATVLVVEPDGTKREHTYWSLTFGPQAGDEGRDFA
EWRELVLETLTRAVRRRLVADVPVGVLLSGGLDSSLITALLAENGQSGLKTFSIGFETVG
DERGDEFQYSDIVARHFGTDHHRLFVDSARALPELTDCVRAMSEPMVSHDNIGFFLLSQE
VAKQVKVVQSGQGADEVFGGYHWYPPLLESRDAVGDYARVFFDRDHAEMAEALDPRFIGP
RFIEDDHSRRWLEQRFAMPGAERPVDKALRLDTTVMLVDDPVKRVDNMTMAAGLEARVPF
LDHELVELAARIPAEMKLPDGGKYVLKEAARSVVPAAVIDRPKGYFPVPALKYLRGPFLE
RVRDVLNAPSARQRGLFRRDYLDRLLADPERHITPLRGSKLWQVALLEFWFQEQGL