Protein Info for MPMX19_04122 in Azospirillum sp. SherDot2

Annotation: Glutathione biosynthesis bifunctional protein GshAB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 586 TIGR03103: GNAT-family acetyltransferase TIGR03103" amino acids 41 to 584 (544 residues), 887.1 bits, see alignment E=2.1e-271 PF00583: Acetyltransf_1" amino acids 161 to 250 (90 residues), 40.7 bits, see alignment E=9.1e-14 PF13508: Acetyltransf_7" amino acids 162 to 251 (90 residues), 35 bits, see alignment E=5.3e-12 PF08445: FR47" amino acids 193 to 253 (61 residues), 30.1 bits, see alignment E=1.4e-10 PF08443: RimK" amino acids 334 to 436 (103 residues), 30.6 bits, see alignment E=8.8e-11 PF02955: GSH-S_ATP" amino acids 350 to 438 (89 residues), 25 bits, see alignment E=4e-09

Best Hits

KEGG orthology group: None (inferred from 70% identity to rce:RC1_3543)

MetaCyc: 59% identical to N-acetylglutaminylglutamine synthase (Sinorhizobium meliloti 1021)
RXN-13455

Predicted SEED Role

"Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30)" in subsystem Cyanophycin Metabolism (EC 6.3.2.29, EC 6.3.2.30)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.29 or 6.3.2.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (586 amino acids)

>MPMX19_04122 Glutathione biosynthesis bifunctional protein GshAB (Azospirillum sp. SherDot2)
MSYARVKPHRLERAASATLRHTRRYTGRPPAGETGADAPRADVVVDCGWGRLIFAHTFDD
LDRMIETLRQEAPGTRDIVFYLQDPHVALSRAPQELFLDPSHTFRLWLTDYRPSRRRPKG
YSVRRLRTREDAEGVQRIYARRRMVPVSSSFLVASRNSRVLTHFVACDDATGEIIGSVTA
VDHAHAYGDPENGSSLWCLAVDPQASFPGIGETLVRTVAEHVQARGRAFLDLSVLHDNES
AIALYEKLNFRRIPAFALKTRNPINERLFTGPQPGEQLNPYATIIVNEARRRGIGVDVLD
AEAGYFCLTWGGRSVVCRESLSELTSAVAMSRCDDKAVTRRVLERASLKVPAQAVAGEPD
ENAAFLATHGRIVIKPARGEQGRGITVDVRDAETMERAIASARQHCDTVLLEQFYEGQDL
RIIVIDKKVVAAAVRRPAEVTGDGSHTVRELIEAQSRRRAAATGGESHIPLDAETDRCLA
AARKSLDDVLAAGEVLPVRKTANLHTGGTIHDVTDSLHPELVEVATVAARALDIPVVGFD
LLVEAPERPGHVFIEANERPGLANHEPQPTAERFVDMLFPQTRGVL