Protein Info for MPMX19_03990 in Azospirillum sp. SherDot2

Annotation: Mannonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 TIGR00695: mannonate dehydratase" amino acids 1 to 390 (390 residues), 484.4 bits, see alignment E=1.4e-149 PF03786: UxuA" amino acids 1 to 388 (388 residues), 386.1 bits, see alignment E=1.3e-119 PF01261: AP_endonuc_2" amino acids 203 to 309 (107 residues), 37.5 bits, see alignment E=2.3e-13

Best Hits

Swiss-Prot: 56% identical to UXUA_SACD2: Mannonate dehydratase (uxuA) from Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)

KEGG orthology group: K01686, mannonate dehydratase [EC: 4.2.1.8] (inferred from 96% identity to azl:AZL_a08380)

MetaCyc: 52% identical to D-mannonate dehydratase (Escherichia coli K-12 substr. MG1655)
Mannonate dehydratase. [EC: 4.2.1.8]

Predicted SEED Role

"Mannonate dehydratase (EC 4.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.8

Use Curated BLAST to search for 4.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>MPMX19_03990 Mannonate dehydratase (Azospirillum sp. SherDot2)
MQETWRWFGPDDPVTLEKARQAGATGIVTALHHMNQGSVWTPEEIQKRRAMIEAAGLTWD
VVESIGVGEEIKTRTGDFQTKIDNYKQSIRNVARAGVPVICYNFMVITDWSRTNLMHRLP
NGGYALRYDSVDFAAYDLFVLKRRNAEASYDPAHVTAAKARFEAMTVEQVAELERNLIDW
LPARDFAYDRDSFRGMLELYQEIGVEDLRANLIEFLREITPVAEEEGVRLCIHPDDPSFP
IFGLPRVMSTAEDVRALFAAVPQEACGLTLCTGSFGSNPKNDLVAMAKEFAPRIHFVHLR
NTTHEPDGSFYEADHLGGDTDLIGVAAAIIDEEARRRQAGRADAQIPMRPDHGHLLGDDI
GQTMNPGYSFVGRLKGLAELRGVMRTIEAFRDKRVA