Protein Info for MPMX19_03973 in Azospirillum sp. SherDot2

Annotation: Esterase YbfF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 PF00561: Abhydrolase_1" amino acids 21 to 249 (229 residues), 88.9 bits, see alignment E=1.1e-28 PF12697: Abhydrolase_6" amino acids 23 to 254 (232 residues), 88.6 bits, see alignment E=2.5e-28 PF12146: Hydrolase_4" amino acids 23 to 248 (226 residues), 44.1 bits, see alignment E=3.9e-15 PF00756: Esterase" amino acids 86 to 112 (27 residues), 23.5 bits, see alignment (E = 1.1e-08)

Best Hits

Swiss-Prot: 46% identical to ABHDB_MOUSE: Protein ABHD11 (Abhd11) from Mus musculus

KEGG orthology group: K01175, [EC: 3.1.-.-] (inferred from 94% identity to azl:AZL_a02710)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>MPMX19_03973 Esterase YbfF (Azospirillum sp. SherDot2)
MPQQALPLTYLEAGEANGGTPMLVLHGLFGSARNWQTLARRFGESRRVYALDLRNHGGAP
WAEGMSYPEMAADVLRFLDDRGFARATIVGHSMGGKTAMALALAHPDRVERLVVADIAPV
SYTHTHAPFVAAMKAAKLEGATRRPEVEAQLADAVPEAPLRSFLMQNLVLENGAFSWRIN
LDSIATNMTGLIGFPDFGGARYDGPALFIGGGASDYIQPEHHDAIRRHFPKADIRMIDGV
GHWLHAERPAEFAAMVEGFL