Protein Info for MPMX19_03972 in Azospirillum sp. SherDot2

Annotation: Enolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01060: phosphopyruvate hydratase
  • amino acids 4 to 421 (418 residues), 684.2 bits, E=3e-210
• PF03952: Enolase_N
  • amino acids 4 to 134 (131 residues), 206.6 bits, E=2.1e-65
• PF00113: Enolase_C
  • amino acids 141 to 423 (283 residues), 438.9 bits, E=1.4e-135
• PF13378: MR_MLE_C
  • amino acids 228 to 392 (165 residues), 29.8 bits, E=7.2e-11

Best Hits

Swiss-Prot: 81% identical to ENO_RHOCS: Enolase (eno) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01689, enolase [EC: 4.2.1.11] (inferred from 96% identity to azl:AZL_b03090)

MetaCyc: 64% identical to enolase subunit (Streptococcus mutans)
Phosphopyruvate hydratase. [EC: 4.2.1.11]

Predicted SEED Role

"Enolase (EC 4.2.1.11)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Serine-glyoxylate cycle (EC 4.2.1.11)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• homolactic fermentation (12/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• glycolysis III (from glucose) (11/11 steps found)
• Rubisco shunt (10/10 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• superpathway of glucose and xylose degradation (15/17 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• Bifidobacterium shunt (13/15 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• heterolactic fermentation (15/18 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• chorismate biosynthesis I (7/7 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• gluconeogenesis III (10/12 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• superpathway of N-acetylneuraminate degradation (17/22 steps found)
• glycolysis IV (8/10 steps found)
• gluconeogenesis I (10/13 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• glycolysis VI (from fructose) (8/11 steps found)
• gallate biosynthesis (2/3 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• quinate degradation I (1/3 steps found)
• quinate degradation II (1/3 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• glycerol degradation to butanol (10/16 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (11/18 steps found)
• formaldehyde assimilation I (serine pathway) (7/13 steps found)
• superpathway of chorismate metabolism (38/59 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (10/42 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.11

Use Curated BLAST to search for 4.2.1.11

Protein Sequence (425 amino acids)

>MPMX19_03972 Enolase (Azospirillum sp. SherDot2)
MSVITEIHAREILDSRGNPTVEVDVALDSGAFGRAAVPSGASTGAHEAVELRDGDKSRFG
GKGVLKAVESVNGEIYDALAGLDADDQRGIDLAMIDLDGTENKSRLGANAILGVSLAVAR
AAAEDAGLPLYRYVGGAFASLLPVPMMNIINGGAHADNPIDIQEFMIMPVGAETGADAIR
MGSEIFQSLKKKLKDAGHNTNVGDEGGFAPNIGSTDEALGFVMKAIEAAGYKPGDDVMLA
LDAASTEFFKNGKYELAGEGKSLSPEQMVAYWADLAGRYPIISIEDGMAEDDWEGWKALT
DAIGNKVQLVGDDLFVTNPKRLAQGIRQGVANSILVKVNQIGTLSETLEAVDMAHKAGYT
AVLSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEQLGVAARFAGR
GILKA