Protein Info for MPMX19_03852 in Azospirillum sp. SherDot2

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 PF21226: MalQ_N" amino acids 65 to 168 (104 residues), 46.7 bits, see alignment E=3.4e-16 TIGR00217: 4-alpha-glucanotransferase" amino acids 135 to 720 (586 residues), 430.5 bits, see alignment E=6.3e-133 PF02446: Glyco_hydro_77" amino acids 188 to 704 (517 residues), 367.3 bits, see alignment E=1.3e-113

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 87% identity to azl:AZL_a00430)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.25

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (729 amino acids)

>MPMX19_03852 4-alpha-glucanotransferase (Azospirillum sp. SherDot2)
MSDLDRLADLLGLEPHYHDIWGNRRETSAATKRALIAAMGLQATTDEEVADSLRTVERRS
WSRPLPPVLIGGEGQGIGLEVALPAGLDEAALVWELTPEGGSAQGGRVAVRDLPVTDRTA
LDGTAYERRALGALLPVSLPIGYHRLDLHVEAAGGAGPSGSTTLIVAPERCVTVEEMVPA
GRSWGIGLQVYSLRSEHDWGMGDFAGLAGFAEVAGRLGAGLVGLSPLHALFPADPNHIGP
YSPSSRQFLNILYIDPAAVPEFAGADDLQARIADPAFQAALASARQAELVDYPAVAALKL
PLLEALHARFRSLPDDHPRKVAYAAFRQDMGAALDRHALFDALHEHFFRQDSAQWMWRSW
PAAFQDPQSAEVADFAATHADRVDFFAWAQFEADRQLGEAAARGRSAGLAMGFYRDLAVA
AHPGGGSAWADPAMLVQGANVGAPPDQFNMNGQNWGLAPLSPLGLRESAYRPFIELLRAN
MRHAAALRIDHVMALQHLFWIPEDGSPGAYVAYPFHDLLRIVALESRRNRCVVIGEDLGT
VPDSFRPALEQAGILSYRVLYFERTADGGFKSPGEYPAGSMATVSTHDLAPFKGFWTGRD
LDWRKRLGLYPDDASRDKDRWDRGVDRWRLLQALSREGLRPDSYPTEDGDQPFRVELSAA
VHAYVSRTPSRIVMVQIEDALADDEQPNLPGTVDQHPNWRRRLPRRLEELEADEDLRHIV
APMTGIAPR