Protein Info for MPMX19_03849 in Azospirillum sp. SherDot2

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 766 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 141 to 761 (621 residues), 980.7 bits, see alignment E=1.5e-299 PF02922: CBM_48" amino acids 156 to 240 (85 residues), 87 bits, see alignment E=1.3e-28 PF00128: Alpha-amylase" amino acids 311 to 381 (71 residues), 28.9 bits, see alignment E=1.4e-10 PF02806: Alpha-amylase_C" amino acids 664 to 763 (100 residues), 87.3 bits, see alignment E=1.1e-28

Best Hits

Swiss-Prot: 66% identical to GLGB_RUBXD: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 93% identity to azl:AZL_a00400)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (766 amino acids)

>MPMX19_03849 1,4-alpha-glucan branching enzyme GlgB (Azospirillum sp. SherDot2)
MASDMTTDTRKDQAQTGVSESRTPEQRAADHRAEALRAAADAIVRADHGDPFAVLGMQQE
APGRPVEVRAFVPGAEKLWVIDSATGEPAGEAERIHQDGFYLAVMPDRTERFRYRLRAQY
PLATQEFEDVYRFGNMLGELDVHLLAEGTHLRNYEKLGAHPREVDGVAGVAFAVWAPSAR
SVSVVGDFNNWDGRRMPMRRRVEVGVWEIFVPGAHAGQRYKFEIRGAGGNLMPAKADPYA
FQAEMRPATASVVHGLSSYDWRDQDWQSRKLAASERTAPISIYEVHLGSWARVPEEDNRF
LTYQELAERLIPYAKDMGFTHIELLPITEHPFDGSWGYQPIGLYAPTARHGSPEDFKDFV
NACHRAGLGVLLDWVPGHFPTDPHGLGDFDGTHLYEHADPRQGFHQDWNTLIYNFGRTEV
QNFLLGNALFWLDQYKLDGLRVDAVASMLYLDYSRKEGEWVPNKFGGRENLESIAFLKRM
NELVYGQQPGAMTVAEESTSWPMVSKPTYLGGLGFGYKWNMGWMHDTLHYMQSDPIHRRY
HHHEMTFGLIYQFSENFVLPLSHDEVVHGKGSLINKMPGDDWQKFANLRAYYGFMFAHPG
KKLLFMGGEFAQWQEWSEARSLDWHLLDQPMHRGMRDLVRDLNGLYRELEPLHKTDCDPS
GFEWIESNDNENSVFTFLRKADESGHIVIAVCNFTPVPRQGYRVGVPLPGRYVERMNTDA
AIYGGSGVGNPGGGVYAEEMPWHGRTHSLDLTIPPLSTLILERKAE