Protein Info for MPMX19_03616 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 5 to 127 (123 residues), 141.9 bits, see alignment E=2.2e-45 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 6 to 126 (121 residues), 128.3 bits, see alignment E=2.3e-41 PF00381: PTS-HPr" amino acids 176 to 255 (80 residues), 67.7 bits, see alignment E=1.9e-22 TIGR01003: phosphocarrier, HPr family" amino acids 179 to 254 (76 residues), 56.1 bits, see alignment E=4.3e-19 PF05524: PEP-utilisers_N" amino acids 307 to 421 (115 residues), 70.3 bits, see alignment E=4.2e-23 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 362 to 856 (495 residues), 434.7 bits, see alignment E=7.3e-134 PF00391: PEP-utilizers" amino acids 449 to 521 (73 residues), 57.9 bits, see alignment E=1.6e-19 PF02896: PEP-utilizers_C" amino acids 553 to 831 (279 residues), 330.7 bits, see alignment E=1.8e-102

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 87% identity to azl:AZL_b04990)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphocarrier protein of PTS system / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Mannitol Utilization or Fructose utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (863 amino acids)

>MPMX19_03616 hypothetical protein (Azospirillum sp. SherDot2)
MTAITLAAPLAGWAMPLSEVPDPAFAQGLVGTGMAIDPTVNELRSPCDGTVLSVHRARHA
CTVRTASGAEILLHLGVDTVGLNGEGFTAHVQDGQSVKTGDRLISFDMDLVGSRAQSLVS
MMVVVNDGYTVDGLSVDREIAAGDAAMTVRGGSGADAGLPAAGPDSGEDGSGETGERSVV
LPIASGLHARPAAALVAATKAYPGTVTIHCRDRRANAKSVVALMGLGTMLGDRLTVRAAG
PGAQDMAESIAVLIEGGLGDPVVAGVAAGAGSQAAPVAPPAAPAPAQPAAEEEVAPPFAP
GEEVLLKGTIAVPGQAVGAVVRRFRSAVRVVEQGAGPDIEEPRLRRALEQVVAGLAQSAQ
RMPEHAAIFQAHRELLDDPELLDGALADIRAGKSAEWSWQRTARLQADALAALADPRMAE
RAADLRDLEQQVLTALSGKGPDVGLAELPAGSIVLADEILPSDLAGVPAGRLAGIGMAHG
GPTSHAVILAAALGVPTVVALGRQAERVPDGAPVVIDGNRGELLVFPPEDTLAATRTAVA
ARTARREENRLTSREECRMADGTRIEVFANLGRVSDAPGAVVEGAEGCGLLRTEFLFLER
QSAPTEDEQYQQYQQIADALEGRPLVIRTLDVGGDKPLSYLPLPKEENPVLGLRGVRVGL
REPELLRAQIRAILRVKPVGVCRIMVPMIASPSELQAVRAMVDEERAKLGRTEPIELGAM
IEVPAAALIADRIGAVADFLSVGTNDLTQYVLAMDRGNPHVAAQLDALHPGVLRLIRLAV
MGAKTHDRVVAVCGGSASDPMAAPLLIGLGVTELSATPAVIADLKAFIRTLRMEDCRRVA
EAALGTDSADEVRRLVAESWPAL