Protein Info for MPMX19_03564 in Azospirillum sp. SherDot2

Annotation: Propane 2-monooxygenase, hydroxylase component large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 64% identical to PRMA_GORST: Propane 2-monooxygenase, hydroxylase component large subunit (prmA) from Gordonia sp. (strain TY-5)

KEGG orthology group: K08684, methane monooxygenase [EC: 1.14.13.25] (inferred from 98% identity to azl:AZL_c02890)

MetaCyc: 64% identical to propane 2-monooxygenase catalytic component large subunit (Gordonia sp. TY-5)
RXN-17568 [EC: 1.14.13.227]

Predicted SEED Role

"Methane monooxygenase component A alpha chain (EC 1.14.13.25)" (EC 1.14.13.25)

MetaCyc Pathways

superpathway of C1 compounds oxidation to CO₂ (9/12 steps found)
methane oxidation to methanol I (1/1 steps found)
nitrite-dependent anaerobic methane oxidation (2/3 steps found)
propane degradation II (2/5 steps found)
propane degradation I (1/4 steps found)

KEGG Metabolic Maps

Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.25

Use Curated BLAST to search for 1.14.13.227 or 1.14.13.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (554 amino acids)

>MPMX19_03564 Propane 2-monooxygenase, hydroxylase component large subunit (Azospirillum sp. SherDot2)
MPDGLTLNKITSQKGIGITEAARRVADLGWTPSYVKEAMSFPTDYKISKAPRDPMKQVLR
SYFPMQEEKDNRVYGALDAALRGDMFRNVEPRWIEWMKLFLAIIPFPEISAARSMATLGQ
LAPGDDLRTGFTMQMIDEFRHSTIQMNLKKWYMENYIDPAGFDITEKAFGKCYATTIGRQ
FGEAFLTGDAITASNIYLQVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRH
IGNGHSMLMSVLKEPDNHLLLERDIRYSFWQNHMIVDAAIGTIIEYGTKHRDKNKESYAE
LWHRWIFEDYYRTYLLPLEKYGIKIHHDDVHEAFDSIVKKNYVHKIAQFFSAGWWANFWR
IEAMTERDFEWFESKYPGWYDEFGVWWENYAKLSKPGSVPITFADTGYVYPHRCWSSLVP
CVIREDFTTDEVDGELFTYASEVDRWTHKEAFAAEYQGRPTPAMGRFSGRRQWEEVYDGW
DLADAIQDMGFVRPDGKTLVAQPHLSFEEKDMWTLDHVRGHTIRSPLLALREMTPQQRQT
HVDEYRKGFKIRRV