Protein Info for MPMX19_03515 in Azospirillum sp. SherDot2

Annotation: Hydrogenase-4 component B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 675 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 36 to 59 (24 residues), see Phobius details amino acids 80 to 101 (22 residues), see Phobius details amino acids 110 to 129 (20 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 165 to 186 (22 residues), see Phobius details amino acids 209 to 231 (23 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 275 to 298 (24 residues), see Phobius details amino acids 310 to 331 (22 residues), see Phobius details amino acids 344 to 366 (23 residues), see Phobius details amino acids 382 to 403 (22 residues), see Phobius details amino acids 429 to 454 (26 residues), see Phobius details amino acids 474 to 498 (25 residues), see Phobius details amino acids 504 to 524 (21 residues), see Phobius details amino acids 535 to 556 (22 residues), see Phobius details amino acids 579 to 598 (20 residues), see Phobius details amino acids 656 to 674 (19 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 131 to 420 (290 residues), 173.4 bits, see alignment E=3.3e-55

Best Hits

KEGG orthology group: K12137, hydrogenase-4 component B [EC: 1.-.-.-] (inferred from 96% identity to azl:AZL_b04420)

Predicted SEED Role

"Hydrogenase-4 component B (EC 1.-.-.-) / Formate hydrogenlyase subunit 3" in subsystem Formate hydrogenase (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (675 amino acids)

>MPMX19_03515 Hydrogenase-4 component B (Azospirillum sp. SherDot2)
MIPLSLLIMSLLLSCGGAILCLLLKHREGDIRLGGSGVGIAAALAGLGAGLTAIAAGQPA
VLDAAGPYPFAHFVLRLDPLAGLMIAVISLLSLVAWIYGFAYVREYAGRGVGAMGFFMNA
FIASMMLVVAADNAFWFLIFFEMMSLASYFLVIFDQDDEAVGAGFLYFLVAHGGSVLIMA
AFFLMANQAGSFDFAAFRAHPLPAPLDSVAFLLAFIGFGAKAGMIPLHIWLPRAHPAAPS
HASALMSGVMIKIGVFGIVKVGIDLLGASAGPAMLGWGLLVLAFGAVSSVLGVVYALAEH
DIKKLLAYHSVENIGIILLGVGTGMIGMALHQPVLAVLGLMAGLYHLLNHAVFKGLLFLG
AGSAIFRMHTKDMEQMGGLARTMPWTALSFLVGALAISAIPPLNGFVSEWYTYQALFAAA
LDGTPLVKFAAPIAAVMLALTGALAVMCFVKAYGIMFAGAPRSHHAEEAREAPAAMVAGM
AILAVACVALGLLAPLVAPVMGRIAAATIGTAPVTLAVGATLLPGDARQATLSTPLLSIL
LIAMAFLPIALKAAFAAKRGGDRKVAAPWAAGYLPDHHMPASAGSFAQPIRMFFAPLYTM
RRAIAGRWTGIALGFERVVTLARRTEPLADRSVIAPIAGAIDASGKWLQIIQAGDFRIYC
LYIVAALIALLALAV