Protein Info for MPMX19_03513 in Azospirillum sp. SherDot2

Annotation: Na(+)/H(+) antiporter subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 38 to 58 (21 residues), see Phobius details amino acids 65 to 82 (18 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details amino acids 147 to 164 (18 residues), see Phobius details amino acids 176 to 197 (22 residues), see Phobius details amino acids 202 to 227 (26 residues), see Phobius details amino acids 248 to 266 (19 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 307 to 329 (23 residues), see Phobius details amino acids 345 to 367 (23 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details amino acids 418 to 440 (23 residues), see Phobius details amino acids 463 to 486 (24 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 78 to 124 (47 residues), 24.5 bits, see alignment 2e-09 PF00361: Proton_antipo_M" amino acids 147 to 426 (280 residues), 194 bits, see alignment E=3.5e-61

Best Hits

Swiss-Prot: 60% identical to HYFD_ECOLI: Hydrogenase-4 component D (hyfD) from Escherichia coli (strain K12)

KEGG orthology group: K12139, hydrogenase-4 component D [EC: 1.-.-.-] (inferred from 98% identity to azl:AZL_b04400)

Predicted SEED Role

"Hydrogenase-4 component D"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>MPMX19_03513 Na(+)/H(+) antiporter subunit A (Azospirillum sp. SherDot2)
MITLPTEPLGLLALAAILVPFGGAAAIAASERASAKWLCQGFAAATVAVIAILAMSLLGD
GKIGGTYELISFGSVSILGLVVDSVSVLIALAVIAIGLLVAVYSAAYLNAGNREHPDIGR
RRFYAFLLVFIGAMTGLVFSSTVVGQLAFFELTGACSWALIGYYESPKALRSAAKALLVT
HVASLGLYAAAALLFLSTGTFALTAIADLAPGMKAAVLLAILVAAWGKSAQLPFHMWLPD
AMEAPTPVSAYLHAASMVKVGVYIFARGLMSSGGAPEIVGWVGSIMAVLTLVYGFFMYLP
QVDLKRLLAYSTITQLAYILLALSLSVFGSDLAFKGAIAHIFNHAFAKTLFFLVAGALSY
TAGTRLLPSLRGIVTKSPLLGVAFVCAALAITGVPPFNGFFSKFAIFAGGFEVGAHHWAL
MLLVVIALAESVGSFAWFLKWLGHSVPGKPSATMAAAAPLPAGMKFVLVALVVMTVVSSS
IAASWLG