Protein Info for MPMX19_03479 in Azospirillum sp. SherDot2

Annotation: Bifunctional NMN adenylyltransferase/Nudix hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01467: CTP_transf_like
  • amino acids 35 to 85 (51 residues), 35.9 bits, 8e-13
• TIGR00125: cytidyltransferase-like domain
  • amino acids 35 to 79 (45 residues), 40.5 bits, 1.2e-14
• PF00293: NUDIX
  • amino acids 240 to 370 (131 residues), 59.9 bits, E=2.8e-20

Best Hits

KEGG orthology group: K13522, bifunctional NMN adenylyltransferase/nudix hydrolase [EC: 2.7.7.1 3.6.1.-] (inferred from 82% identity to azl:AZL_d04310)

Predicted SEED Role

"Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)" in subsystem NAD and NADP cofactor biosynthesis global or Nudix proteins (nucleoside triphosphate hydrolases) or NAD regulation (EC 2.7.7.1, EC 3.6.1.13)

MetaCyc Pathways

• NAD biosynthesis from nicotinamide (1/2 steps found)
• NAD salvage pathway IV (from nicotinamide riboside) (1/2 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.13

Use Curated BLAST to search for 2.7.7.1 or 3.6.1.- or 3.6.1.13

Protein Sequence (377 amino acids)

>MPMX19_03479 Bifunctional NMN adenylyltransferase/Nudix hydrolase (Azospirillum sp. SherDot2)
MTIVSPTGRESGALCLPQKTVAPGAQGASGFDFLVFVGRFRPFHLGHLTVMKRALSLGRH
LIVLVGSSRQARSHRNPFRFEEVRSCIEGAFTPEECGRITVLPLIDRYNDLEWIRDVQAA
VHGVVVQHHRPADGSPPKVGLIGHSKDHSSYYLRMFPQWGAVECANFQGISATPIRDAYF
ADAAAALEEWKEALPATVAAFLAEFAHTDEYKRLAEESAYIAKYKAGWANAPYPPTFLTA
DAVVVLSGHVLLVERRGLPGRGLLALPGGFVGQHERIKDAMIRELREETKLKIPAGFLEG
GIRASQIFDHPYRSTRGRTVTQAFLIQLKPTDEGFPKVRGGDDARSARWMPIADIDPERM
FEDHYHIIQTLIALSNN