Protein Info for MPMX19_03478 in Azospirillum sp. SherDot2

Annotation: Glutamine-dependent NAD(+) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 12 to 239 (228 residues), 82.2 bits, E=3.9e-27
• PF02540: NAD_synthase
  • amino acids 277 to 543 (267 residues), 209.3 bits, E=5.2e-66
• TIGR00552: NAD+ synthetase
  • amino acids 284 to 545 (262 residues), 170.3 bits, E=2.2e-54

Best Hits

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 90% identity to azl:AZL_d04320)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD de novo biosynthesis III (6/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• NAD de novo biosynthesis II (from tryptophan) (6/9 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.5

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (569 amino acids)

>MPMX19_03478 Glutamine-dependent NAD(+) synthetase (Azospirillum sp. SherDot2)
MSNTTSSLRIVCVQANPTVGALQGNFDIVRRLRAQYRGKADLLVFSECFGSGYPLQDLVL
RPGFRRDFRAALDALAGEMRGDGGPAVLVGGPLDGAALPYNAAFLIETDGSMKIVLKHTL
PNDEVYDEKRVFAPGPLPSPVDFRGFRLGIAICEDFWHGKVAQALAAEGAEILIVPNGSH
FRTGKQAVRLAIGRRTVKATGLPVLYVNQVGGQDSLVFDGGSYAMDRAGLVIAQAGFREC
EFDIALERGEDGGVDLRRGDVLGMLPNGYPDETEAIYRALVLGLRDYVDKNGFPGVVLGM
SGGIDSALSAAVAVDALGAARVLPVRMPSPYTSAESMEDAERAASLLGTRLLTVPIAPAM
AAFDGMLAPVFDGLPVPAADTTFENVQARARGMTLMALSNRLGLMVLSTGNKSEMSVGYA
TLYGDMCGGYSVLKDVYKTIVFRLARWRNTHRAEGLLGPQGAVMPDRIIAKPPSAELREG
QTDEQALGAYEHLDAVLATMVEGLNGADRAAELASAAVGQPISTAYAERIARLTARAQYK
RDQSPPGVVVTERTYGPGWRLPVTNHYGL