Protein Info for MPMX19_03463 in Azospirillum sp. SherDot2

Annotation: Butyrate--acetoacetate CoA-transferase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02428: 3-oxoacid CoA-transferase, B subunit
  • amino acids 4 to 207 (204 residues), 298.4 bits, E=1.1e-93
• PF01144: CoA_trans
  • amino acids 7 to 199 (193 residues), 145 bits, E=1.2e-46

Best Hits

Swiss-Prot: 54% identical to CTFB_CLOAB: Butyrate--acetoacetate CoA-transferase subunit B (ctfB) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

KEGG orthology group: K01036, butyrate-acetoacetate CoA-transferase [EC: 2.8.3.9] (inferred from 77% identity to rpc:RPC_2004)

MetaCyc: 54% identical to butyrate-acetoacetate CoA-transferase, subunit B (Clostridium acetobutylicum ATCC 824)
Acetoacetyl-CoA hydrolase. [EC: 3.1.2.11]; Butyrate--acetoacetate CoA-transferase. [EC: 3.1.2.11, 2.8.3.9]; 2.8.3.9 [EC: 3.1.2.11, 2.8.3.9]

Predicted SEED Role

"Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism (EC 2.8.3.9)

MetaCyc Pathways

• ketolysis (3/3 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• pyruvate fermentation to acetone (4/5 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• isopropanol biosynthesis (engineered) (3/5 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (8/13 steps found)
• L-lysine fermentation to acetate and butanoate (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of L-lysine degradation (13/43 steps found)

KEGG Metabolic Maps

• Butanoate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.9

Use Curated BLAST to search for 2.8.3.9 or 3.1.2.11

Protein Sequence (218 amino acids)

>MPMX19_03463 Butyrate--acetoacetate CoA-transferase subunit B (Azospirillum sp. SherDot2)
MDEKTLIAKRVALELRPGNLVNLGIGLPTMVAAHLPAGVNVFFQSENGIVGMAPVPEPGA
ENEDLSDAGGSPIGAVPGAASFDSAMSFGLIRGGHLDVTVLGGLQIDEEGRLANWMVPGK
MVPGMGGAMDLVSGAKRVIVAMQHSAKGTPKIVKRCELPLTSVRRVDLIVTELAVIEPTD
KGLVLREVAPSVTVADVLAQTGAELIVPSAVPTMPVAA