Protein Info for MPMX19_03382 in Azospirillum sp. SherDot2

Annotation: Biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 3 to 111 (109 residues), 139.8 bits, E=1.2e-44
• PF02786: CPSase_L_D2
  • amino acids 116 to 323 (208 residues), 243.4 bits, E=4.5e-76
• PF07478: Dala_Dala_lig_C
  • amino acids 147 to 292 (146 residues), 29 bits, E=1.8e-10
• PF02785: Biotin_carb_C
  • amino acids 337 to 443 (107 residues), 123.9 bits, E=7.6e-40

Best Hits

Swiss-Prot: 50% identical to ACCC1_BACSU: Biotin carboxylase 1 (accC1) from Bacillus subtilis (strain 168)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 93% identity to azl:AZL_a02440)

MetaCyc: 51% identical to biotin carboxylase (Arabidopsis thaliana col)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• jadomycin biosynthesis (3/9 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Protein Sequence (459 amino acids)

>MPMX19_03382 Biotin carboxylase (Azospirillum sp. SherDot2)
MTIRRLFVANRGEIAVRIIRAAKSLGIETVQAYSEADREMLAVKLADQAVCVGRPAARHS
YLDAAGLVAAARTAGCDAVHPGYGFLAENAEFADMVESFGMVFVGPKGETIRRMGDKAAA
REAAIAAGVPVVPGSQGRVADIDAALAAAGTIGYPVMIKAAAGGGGRGIRIAETPDELRR
LAPQAQSEAQAAFGDGGLYLERVIGNARHIEVQILGDGERAIHCFERECSLQRRRQKVWE
EAPAACLDEATRAQICASAVALAESVGYRGAGTLEYLYDEPSGAFYFIEMNTRIQVEHPV
TEMITGIDLVAAMIRIAGGEPLPVTQDRVTRTGHAIEVRINAEDPAAGFLPCPGTVERLI
VPTAPAIRFDGMIYEGYTVPPFYDSLLGKLIVHGATRDEAIDRLADALEGFAVTGLKTTV
PLHKALAADPDVRAGKAHTRFLEGWLAGNPAFNSQKAAQ