Protein Info for MPMX19_03142 in Azospirillum sp. SherDot2

Annotation: Putative thiamine pyrophosphate-containing protein YdaP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 4 to 120 (117 residues), 109.7 bits, E=1.1e-35
• PF00205: TPP_enzyme_M
  • amino acids 200 to 329 (130 residues), 90.1 bits, E=1.6e-29
• PF02775: TPP_enzyme_C
  • amino acids 389 to 543 (155 residues), 98.8 bits, E=4e-32

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 71% identity to noc:Noc_0471)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

• pyruvate to cytochrome bd oxidase electron transfer (1/2 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Protein Sequence (600 amino acids)

>MPMX19_03142 Putative thiamine pyrophosphate-containing protein YdaP (Azospirillum sp. SherDot2)
MAPNVSDFFWQRLSEWGVKRVFGYPGDGINGLVGALARAGDRFEFIQPRHEELAAFMATA
HAKYTGEMGVCLATSGPGAIHLLNGLYDAKLDHQPVLAIVGQQALTALGGQYQQEVDLVS
LFKDVAGEYVQMASTPAQVRHIVDRAVRIAKAERTVTCIIMPNDLQEMDAVEAPARAHGT
IHSGVGWSAPVVRPAAPDLRHAADVLNAGNKVAILVGAGALNATDEVIEIADILGAGVAK
ALLGKAALPDDLPFVTGSIGLLGTRPSYEMMVNCDTLLMIGSGFPYSEFLPKEGQARGVQ
IDIDPKMLSIRYPMEVNLVGDSAGTLRDLIPLLQRKQDRSWRERIEAGVADWWTVLEARA
MNDATPLNPQRVFWELSPRLPDDVVIACDTGSGTNWYARDIKIRRGMKATLCGGLATMGP
GMPYALAAKFAHPDRPVLAIVGDGAMQMNGMNALITLSKYCKQWSDPRFVVLVLNNRDLN
QVTWEMRAQNGDPKFEASQELPDFPYARYAELLGFKGLFVDKPENLAAVWDEAMATRGPV
LVEAYTDPNVPPLPPHITLKNAAAMMATLAKGDPEVWDMLKDTAKDLVQSYLPHGGGKKG