Protein Info for MPMX19_02940 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01257: 2Fe-2S_thioredx
  • amino acids 52 to 184 (133 residues), 81.5 bits, E=1.2e-26
• PF01512: Complex1_51K
  • amino acids 229 to 389 (161 residues), 133.8 bits, E=9.2e-43
• PF10531: SLBB
  • amino acids 414 to 463 (50 residues), 29 bits, 1.5e-10
• PF10589: NADH_4Fe-4S
  • amino acids 501 to 578 (78 residues), 75.9 bits, E=3.7e-25

Best Hits

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 66% identity to mpo:Mpop_0294)

MetaCyc: 66% identical to NAD-dependent formate dehydrogenase beta subunit (Methylorubrum extorquens AM1)
1.2.1.2-RXN [EC: 1.17.1.9]

Predicted SEED Role

"tungsten-containing formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

MetaCyc Pathways

• superpathway of C1 compounds oxidation to CO₂ (9/12 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• oxalate degradation VI (2/4 steps found)
• oxalate degradation III (2/5 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.17.1.9 or 1.6.5.3

Protein Sequence (595 amino acids)

>MPMX19_02940 hypothetical protein (Azospirillum sp. SherDot2)
MTAFPPIISATPPGGVPARSIHPGSGRRKTRDQQKGRQVDPLSLDEIRALLGDRSRQPDL
LIEHLHLLQDHYGCLHARHLAALAKEMKLALVEVFEVASFYAHFDIVMDGEPAPPKLTIR
VCDSLSCALAGAEQLHEALKAGVDERDVRVVRAPCMGGCNNAPVVAIGHAPHENATVESV
LDAVAHGHVHPDIPDYLGFDDYIRGGGYKMLAECLNGARDVDAVLKGLEDSSIRGLGGAG
FPTGLKWRYVRAEPGPRMMAINGDEGEPGTFKDRFHLERDPHRFLEGMLIGAWTVEATDV
YIYLRDEYPQCREILLREIPKVEAAGFARHTRIHLRRGAGAYICGEESAMLESIEGKRGL
PRHKPPFPSVVGLFGRPTLINNIETVFWVREILEKGGAWFASHGLNGRKGLRTFSVSGRV
KDPGVKLAPAGITLQELIDQYCGGMADGHTLKGYLPGGPSGGILPAAMANIPLDFGTLEA
HGSFIGSAAVVVLSDKDNMRDVALNLLKFFEDESCGQCTPCRVGTEKAVRLIGEPEWDEE
LLAALATVMGDASICGLGQAAMNPIKLVMKHFRDDFVSRQGRAQGAAQGATGSQG