Protein Info for MPMX19_02933 in Azospirillum sp. SherDot2

Annotation: Methanol dehydrogenase [cytochrome c] subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 611 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 24 to 566 (543 residues), 741.9 bits, see alignment E=2e-227 PF13570: PQQ_3" amino acids 132 to 161 (30 residues), 19.2 bits, see alignment (E = 2e-07) PF13360: PQQ_2" amino acids 133 to 226 (94 residues), 48 bits, see alignment E=1.9e-16 PF01011: PQQ" amino acids 143 to 175 (33 residues), 31.9 bits, see alignment (E = 1.2e-11)

Best Hits

Swiss-Prot: 64% identical to XOXF_PARDE: Putative dehydrogenase XoxF (xoxF) from Paracoccus denitrificans

KEGG orthology group: None (inferred from 70% identity to smd:Smed_3943)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (611 amino acids)

>MPMX19_02933 Methanol dehydrogenase [cytochrome c] subunit 1 (Azospirillum sp. SherDot2)
MNPFVRCLLVTAAIQAATAAIPTGAFANDDILKNQKDPSNWALQLGNYAGQRYSTLDQIT
RDNVKNLRPVWQFSTGVLRGHEGGPIVVGDTMYISTPFPNNVYALDLNDNGRIKWKYEPR
QDSSVIPVMCCDTVTRGVAVAGNKLFLGQADNTLVALDTETGKVLWSAKNGDASKGETLT
AAPLVVKDKVYVGISGGEFGVRGHLTAYDINSGKMVWRAYSTGPDKDVMIDPQKTMMLGK
PIGEPDLGIKTWPADQWKIGGGTTWGWYTYDPELNLIYYGTGNPGTWNPAQRAVDKDRAK
SDNKWSMTIFARDADTGQAKWVFQKTPFDEWDYDGVNENILADVTIGGGKRKALINFDRN
GFAYTIDRTNGELLVAQKYDPSVNWASEIDMKTGRPKVNDKYSTFAQGEDHNTTGVCPAA
LGSKDQQPASYSPDSGLIYVPTNHICMDYEPFKVEYSAGQPYVGATLSMYPAPDSHGGMG
NFIAWDPSAGKIVWSKPERFAVWSGALSTKGGVGFYGTLEGYLKAFDLKDGKDLYNFKTS
SGIIGNVNTYMHKGKQYIAVLSGVGGWAGIGLAAGLTGEQEGLGAVGAHKALGEYTQLGG
TLTVFALPDTQ