Protein Info for MPMX19_02866 in Azospirillum sp. SherDot2

Annotation: 4-hydroxymandelate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 20 to 356 (337 residues), 385.8 bits, E=3.7e-119
• PF00478: IMPDH
  • amino acids 270 to 311 (42 residues), 28 bits, 2.3e-10
• PF01645: Glu_synthase
  • amino acids 277 to 317 (41 residues), 24.1 bits, 4.1e-09

Best Hits

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 69% identity to rme:Rmet_0839)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• methylglyoxal degradation V (2/3 steps found)
• photorespiration I (6/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3, 1.1.3.15

Use Curated BLAST to search for 1.1.2.3 or 1.1.3.15

Protein Sequence (359 amino acids)

>MPMX19_02866 4-hydroxymandelate oxidase (Azospirillum sp. SherDot2)
MIVPPDTVSLSDYERHFNGRIDPATRAYIAGAGADGITQRANREAFDRLRLMPRVLRNLS
GATARSELFGEVLDYPIVLAPMAHHRLVHPDGELATVEAAGLTRSWMTVSTQASVRVDEI
ARRATAPLWFQLYSQPRREDTLTLVRRAEDAGCKALVLTVDAPVNGMRNMEQRAGFRLPP
TIAPVNLAGLAPDDFRPARPGSPVFQGMLHAAPTWDMVAWLCSETGLPVLLKGILNPEDV
EPAIAAGTGGLIVSNHGGRTLDTLPAGIDALPAVAERAARRLPVLMDGGIRRGTDILKAL
ALGADAVLVGQPVLHALAVGGMAGVAHMLTLLQTELEVAMALTGRPTLPDIDRSVIFGG