Protein Info for MPMX19_02810 in Azospirillum sp. SherDot2

Annotation: D-threonate 4-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 TIGR00557: 4-hydroxythreonine-4-phosphate dehydrogenase PdxA" amino acids 5 to 336 (332 residues), 346.2 bits, see alignment E=1e-107 PF04166: PdxA" amino acids 38 to 332 (295 residues), 337.8 bits, see alignment E=2.9e-105

Best Hits

Swiss-Prot: 76% identical to PDXA2_BRADU: Putative D-threonate 4-phosphate dehydrogenase (pdxA2) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00097, 4-hydroxythreonine-4-phosphate dehydrogenase [EC: 1.1.1.262] (inferred from 97% identity to azl:AZL_b03390)

Predicted SEED Role

"4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.262)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.262

Use Curated BLAST to search for 1.1.1.262

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (344 amino acids)

>MPMX19_02810 D-threonate 4-phosphate dehydrogenase (Azospirillum sp. SherDot2)
MTPHLAITMGDPAGVGPEIIVKACARLKDRLADGTLRLLVIGSNPALHAARDALGSDLDF
PEVTDIDGEWPALACLQAGPEGEPIRPGVLSVDGGRFAYLAVERAVRLAEAGRIHGIVTA
PLNKEAMNKAGYHFAGHTDLLAELTGARGSVMMLAHGNMRVSHVTTHIALEDVPKKLTPE
RLRYVIDRTDETLAGLGLSRRRIAIAALNPHAGEGGLFGRQDIEVTEPVIRQCVADGLDV
VGPVPGDTIFVKLRAGQYDAVVAMYHDQGHIPVKLLGFNVNPETGTWDALSGVNITLGLP
IIRTSVDHGTAFDIAGKGIANELSLIEAIDYAEKLAAARVRVAA