Protein Info for MPMX19_02787 in Azospirillum sp. SherDot2

Annotation: (R)-benzylsuccinyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 8 to 125 (118 residues), 60.8 bits, E=3.2e-20
• PF02770: Acyl-CoA_dh_M
  • amino acids 130 to 225 (96 residues), 76.2 bits, E=3.6e-25
• PF00441: Acyl-CoA_dh_1
  • amino acids 239 to 387 (149 residues), 130.4 bits, E=1.3e-41
• PF08028: Acyl-CoA_dh_2
  • amino acids 254 to 367 (114 residues), 46.7 bits, E=8e-16

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 90% identity to azl:AZL_b02310)

MetaCyc: 44% identical to 4-(phosphooxy)pentanoyl-CoA phosphatase/isomerase (Pseudomonas putida KT2440)
5.4.4.M2 [EC: 5.4.4.M2]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 4-oxopentanoate degradation (4/9 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3 or 5.4.4.M2

Protein Sequence (398 amino acids)

>MPMX19_02787 (R)-benzylsuccinyl-CoA dehydrogenase (Azospirillum sp. SherDot2)
MDFTLPAELEDYRRRVRAFVEDEILPVEADRANWDEHENIAEAPLAALRAKVKAAGLWTL
QLPKEVGGHGLPMVGVAACYEEMNRSIFGPVSFNAAAPDDGNMRLLSMVGTPAQKERWLA
PIVEGRVRSAFAMTEPHPGGGSDPSMMKTRAERKGDRWVVTGRKWFITGADAAQHFILMA
RTSDDPRKGLTAFLFDKDQPGWRIERRIPIMGPEEHGGHCELIFDGLEIPDENVVMKVGD
GLKVTQIRLGPARLTHCMRWTGLAKRCLEIAGAYVKERESFGISLAEHEGVQWMLGETAM
QIEIGRLLTMKAAHALDSGSFARKEVSMAKVQVADTLFKAADTAIQLCGARGYSKDTVLE
WIYRYARQAKLVDGASEVHKMVLSRSYLAEGDDFWGWR