Protein Info for MPMX19_02739 in Azospirillum sp. SherDot2

Annotation: Dihydrofolate synthase/folylpolyglutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 39 to 452 (414 residues), 324.3 bits, E=7.4e-101
• PF08245: Mur_ligase_M
  • amino acids 62 to 279 (218 residues), 35.1 bits, E=6.9e-13

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 90% identity to azl:AZL_027050)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (38/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (456 amino acids)

>MPMX19_02739 Dihydrofolate synthase/folylpolyglutamate synthase (Azospirillum sp. SherDot2)
MSDAPTPLANPSARLAATRSDPVLDRLKGLHPKIIDLSLDRVHRLLAALDHPERKLPPVV
HVAGTNGKGSTVAFLRAMLEAAGHRVHVYTSPHLVRFHERIRLGGRQGGRLIDDDHLAAL
LEECEVANAGNPITFFEVTTVAAFLAFSRVPADVVLLETGLGGRLDATNVVDRPAVTAIT
RISYDHRQFLGDSLLEIASEKAGIFKPGIPVVLAPQPEADALKALNLRANAIAAPIQSWS
VEERPDGFRFESTRRRLDLPMPGLAGAHQITNAGVAIACLDHLPLAVDDEAVRRGLAGVE
WPARLQRLTRGPLAESLPAGWELWLDGGHNDSAGEVLARQAVDWSRGQPDEGSDGGSSLP
LLLIYGMLSSKDPFEFLGPLAPFTHSLRAVAIPGEEASFTAEESCETANLCGIRDHAAAD
GVDAALADLVAGRRTPARVLICGSLYLAGTVLAENG