Protein Info for MPMX19_02715 in Azospirillum sp. SherDot2

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 827 TIGR01059: DNA gyrase, B subunit" amino acids 20 to 827 (808 residues), 954.9 bits, see alignment E=1.1e-291 PF02518: HATPase_c" amino acids 47 to 161 (115 residues), 67.5 bits, see alignment E=4.2e-22 PF00204: DNA_gyraseB" amino acids 245 to 419 (175 residues), 193.5 bits, see alignment E=6.6e-61 PF01751: Toprim" amino acids 447 to 558 (112 residues), 66.5 bits, see alignment E=5.8e-22 PF21249: GyrB_hook" amino acids 560 to 589 (30 residues), 26.5 bits, see alignment (E = 1.2e-09) PF18053: GyrB_insert" amino acids 601 to 725 (125 residues), 29.6 bits, see alignment E=1.8e-10 PF00986: DNA_gyraseB_C" amino acids 755 to 816 (62 residues), 101.2 bits, see alignment 8.3e-33

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 97% identity to azl:AZL_027280)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (827 amino acids)

>MPMX19_02715 DNA gyrase subunit B (Azospirillum sp. SherDot2)
MAQEALKNDLPQSEPQQADYGAESITVLRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVD
NAIDEALAGYCDKVEVVLNADGSVTVRDNGRGIPTDIHSEEGVSAAEVVMTQLHAGGKFN
QNSYKVSGGLHGVGVSVVNALSETLDLRIWRGGRTWTMRFRHGNAEAPLADVGEAPMVPE
KGKPLSGTEVTFMPSTETFTNIEFDFATLEHRLRELAFLNSGVRLLLTDARGVEPKVQDL
HYEGGLEAFVSWLDRSKTALHKPAITLKNERPTEHGGVVTVECALQWNDSYHETTLCFTN
NIPQKDGGTHLAGFRAALTRAINNYATESGIAKKEKVSLSGDDAREGLTCVLSVKVPDPK
FSSQTKDKLVSSEVRPVVEAVVGEALAQYFEEHPADARRVIQKVVEAAAAREAARKARDI
TRKSSLSMSSLPGKLADCQERDPALSELFIVEGDSAGGSAKQGRSRQSQAILPLRGKILN
VERARLDKMLGSAEIGTLIAALGTGISDEFNADKARYHKIIIMTDADVDGSHIRTLLLTF
FFRQMPDLIERGYLYIAQPPLYRIKRGNAKERYLKDDRALEEYLVEAALGDLSVRLSDGS
LLIGEQLAALVEQARTARPAIDTLSRKVGNAMVVEQAAVAGTLSAALADDKAAAEAAAQT
VAERMNGLDADGGWRGEALPQGGYAVIRARRGVTHRHLFDTDLLKSGEARRLDALAADLK
RVFGTAGKAFEKQKESRALTGPVALFDTVMELGRRGVTIQRYKGLGEMNPDQLWETTLDP
TKRSLLQVRVSHVDQAEEVFSTLMGDVVEPRREFIQDNALKVSNLDV