Protein Info for MPMX19_02658 in Azospirillum sp. SherDot2

Annotation: Glutamate synthase [NADPH] small chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 9 to 468 (460 residues), 509.1 bits, E=4.9e-157
• PF14691: Fer4_20
  • amino acids 25 to 136 (112 residues), 131.6 bits, E=3.9e-42
• PF01494: FAD_binding_3
  • amino acids 150 to 182 (33 residues), 22.8 bits, see alignment (E = 1.7e-08)
• PF07992: Pyr_redox_2
  • amino acids 151 to 458 (308 residues), 102 bits, E=1.5e-32
• PF13450: NAD_binding_8
  • amino acids 153 to 186 (34 residues), 38.1 bits, see alignment (E = 5.2e-13)
• PF00070: Pyr_redox
  • amino acids 292 to 364 (73 residues), 23.4 bits, E=2.4e-08

Best Hits

Swiss-Prot: 88% identical to GLTD_AZOBR: Glutamate synthase [NADPH] small chain (gltD) from Azospirillum brasilense

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 96% identity to azl:AZL_001000)

MetaCyc: 88% identical to glutamate synthase beta subunit (Azospirillum brasilense)
Glutamate synthase (NADPH). [EC: 1.4.1.13]

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Protein Sequence (482 amino acids)

>MPMX19_02658 Glutamate synthase [NADPH] small chain (Azospirillum sp. SherDot2)
MANQKMLGFVHTAQKMPDKRSAADRRQDFAEIYARFSNERAGEQANRCSQCGVPFCQVHC
PVSNNIPDWLKLTAEGRLEEAYEVSQATNNFPEICGRICPQDRLCEGNCVIEQSTHGAVT
IGSVEKYINDTAWENGWVKPRKPVRELGLSVGVIGAGPAGLAAAEELRAKGYEVHVYDRH
DRMGGLLVYGIPGFKLEKDVVARRVQRLADAGVIFHSNFEVGRDASLAELRERHVTILIA
TGVYKARDIKAPGSGLKNIVPALEYLTTSNKVGLGDSMEAYEDGSLNAAGKKVVVLGGGD
TAMDCVRTAIRQGATSVKCLYRRDRKNMPGSQREVAHAEEEGVEFVWQAAPEAFTGNDVV
TGVRAVRIHLGVADATGRQTPQVIEGSDFTEPADLVIKALGFEPEDLPGMFGSPDLTVTR
WGTLLVDHRTKMTSLDGVFAAGDIVRGASLVVWAIRDGRDAADAMHAYAQTVGAPKLAVA
AE