Protein Info for MPMX19_02542 in Azospirillum sp. SherDot2

Annotation: Imidazole glycerol phosphate synthase subunit HisF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00735: imidazoleglycerol phosphate synthase, cyclase subunit
  • amino acids 1 to 251 (251 residues), 369.5 bits, E=3.9e-115
• PF00977: His_biosynth
  • amino acids 5 to 234 (230 residues), 299.7 bits, E=6.4e-94

Best Hits

Swiss-Prot: 91% identical to HIS6_AZOBR: Imidazole glycerol phosphate synthase subunit HisF (hisF) from Azospirillum brasilense

KEGG orthology group: K02500, cyclase [EC: 4.1.3.-] (inferred from 97% identity to azl:AZL_028780)

MetaCyc: 52% identical to imidazole-glycerol-phosphate synthase cycloligase subunit (Thermotoga maritima)
GLUTAMIDOTRANS-RXN [EC: 4.3.2.10]

Predicted SEED Role

"Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)" in subsystem Histidine Biosynthesis (EC 4.1.3.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• L-histidine biosynthesis (10/10 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-

Use Curated BLAST to search for 4.1.3.- or 4.3.2.10

Protein Sequence (261 amino acids)

>MPMX19_02542 Imidazole glycerol phosphate synthase subunit HisF (Azospirillum sp. SherDot2)
MLKMRVIPCLDVKDGRVVKGVNFVDLVDAGDPVEQARVYDREGADELTFLDITASHENRD
TIFDVVRRTAEQVFMPLTVGGGVRTVDDIRKLLLAGADKVSINTAAIHRPEFVREGAEKF
GAQCIVVAIDAKKVAPGKWEVFTHGGRNATGIDAVEWARRMESLGAGEILLTSMDRDGTK
SGFDLELTRTVADSIRVPVIASGGVGTLDHLVEGIREGHATAVLAASIFHFGTYTIGQAK
AALATAGIPVRPAKALETTNG