Protein Info for MPMX19_02448 in Azospirillum sp. SherDot2

Annotation: Glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 TIGR01035: glutamyl-tRNA reductase" amino acids 6 to 402 (397 residues), 286 bits, see alignment E=2.6e-89 PF05201: GlutR_N" amino acids 9 to 158 (150 residues), 138 bits, see alignment E=3.4e-44 PF01488: Shikimate_DH" amino acids 174 to 308 (135 residues), 79.8 bits, see alignment E=3.2e-26 PF00745: GlutR_dimer" amino acids 323 to 407 (85 residues), 37.9 bits, see alignment E=2.9e-13

Best Hits

Swiss-Prot: 35% identical to HEM1_PSEFS: Glutamyl-tRNA reductase (hemA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 96% identity to azl:AZL_001400)

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (443 amino acids)

>MPMX19_02448 Glutamyl-tRNA reductase (Azospirillum sp. SherDot2)
MTTTSYLVIGASHRTCSGVVRDRLSTDEAEVAGMLERLRAVGVGQALWLSTCDRVEVQAV
HERPNEAALAIAEVMADRAGLSTSDLAPQLYTRTGIDAVRHLFAVACSLDSQIVGEPHIL
GQLKAAHRSASGAGLTGPELEAVLQAAYAAAKRVRSETPIAEGATSLAAAAIQVARDLHG
DIRRCGALLVGMGDMGALVLEGLREAGLPRLTVAAPVDRRAEAAARRLDGHFAPWADLET
AMTGADILVTAAGLGRYILTAPMMETVLKRRRRRPVFVVDAAIPADVDPAVAGMDGAFVY
DLADLERVALQGRVGREAATQAAWMIVDEAVAAFARDRAERAAVPAVAALRSHFEAERRR
LLAGHADLDAASATRLLVNRLLHAPSEALRALAADGSGEGLAERAAAERLMFRLFGLDRL
TAAALEENERDKDERREPGREVR