Protein Info for MPMX19_02442 in Azospirillum sp. SherDot2

Annotation: Chaperone protein ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 867 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 855 (850 residues), 1431.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 55 bits, see alignment 4.8e-18 amino acids 95 to 146 (52 residues), 41.3 bits, see alignment 9.3e-14 PF00004: AAA" amino acids 205 to 318 (114 residues), 50.2 bits, see alignment E=2.4e-16 amino acids 602 to 720 (119 residues), 33.6 bits, see alignment E=3.3e-11 PF17871: AAA_lid_9" amino acids 344 to 445 (102 residues), 125.4 bits, see alignment E=5.4e-40 PF07724: AAA_2" amino acids 596 to 760 (165 residues), 241.1 bits, see alignment E=4.1e-75 PF07728: AAA_5" amino acids 601 to 722 (122 residues), 52.9 bits, see alignment E=2.7e-17 PF10431: ClpB_D2-small" amino acids 766 to 846 (81 residues), 104 bits, see alignment E=2.4e-33

Best Hits

Swiss-Prot: 73% identical to CLPB_RHOPA: Chaperone protein ClpB (clpB) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 98% identity to azl:AZL_001460)

MetaCyc: 59% identical to disaggregase ClpB (Mycobacterium tuberculosis H37Rv)
RXN185E-10

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (867 amino acids)

>MPMX19_02442 Chaperone protein ClpB (Azospirillum sp. SherDot2)
MDFEQYTERSRGFIQAAQTLAVRRGHQRLTPEHLLKTLLDDKEGLAANLIRAAGGDPKAA
LSAVDAELDKLPKVEGSGAGQLYLTPELSRVFEQAEKVAEKAGDSFVTAERILLALAMAD
GTPSGKALKSAGVTPQALNTAINDIRKGRTADSASAEQGYDALKKYARDLTAAARDGKLD
PVIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVEGLAQRIVKGDVPEGLKNKQLL
SLDLGALVAGAKYRGEFEERLKAVLSEIQAAAGEIVVFIDELHTLVGAGKSDGAMDASNM
LKPALARGELHCVGATTLDEFRKYIEKDAALARRFQPVFVSEPTVEDTISILRGLKERYE
VHHGVRITDSAIVSAATLSNRYITDRFLPDKAIDLIDEAASRLRMAVDSKPEAIDELDRR
IIQLKIEREALKREQDSASRDRLVNLERELSDLEQESAELTAKWQAEKDQLQGAQKIKED
LEKARTELETAQRDGNWGRAGELAYGVIPGLEKALKDAEEHASSRMLNEEVRDSDIAAVV
SRWTGVPVDKMLAGEREKLLAMEDKLRGRVIGQDEAIVAVSNAVRRARAGLQDPNRPIGS
FLFLGPTGVGKTELTKALAEFLFDDETAMVRLDMSEYMEKHSVARMIGAPPGYVGYEEGG
ALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNVVIIMTSNL
GSQALAEQAEGEDSAAVRDEVMEAVRAHFRPEFLNRLDEILLFHRLDRRHMGGIVKIQLG
RLTKMLADREITLTVDEAATQWLADAGYDPVYGARPLKRVIQRELQNPMATLILEGRIKD
GQTVTVGAEGGSLTIDGQPVSGLVRKG