Protein Info for MPMX19_02419 in Azospirillum sp. SherDot2
Annotation: Hippurate hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 40% identical to ILL7_ORYSJ: IAA-amino acid hydrolase ILR1-like 7 (ILL7) from Oryza sativa subsp. japonica
KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 95% identity to azl:AZL_001650)MetaCyc: 39% identical to N-acetyl amino acid acetylase (Bacillus subtilis subtilis 168)
3.5.1.-
Predicted SEED Role
"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)
MetaCyc Pathways
- L-lysine biosynthesis II (7/9 steps found)
- S-(2-succinyl)-L-cysteine degradation (2/4 steps found)
- S-benzyl-L-cysteine degradation (1/5 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Lysine biosynthesis
- Phenylalanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47
Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (393 amino acids)
>MPMX19_02419 Hippurate hydrolase (Azospirillum sp. SherDot2) MPINNRIAAFQDDMTAWRRDIHAHPELGFEEKRTSDIVATKLAEFGIAVHRGLGGTGVVG TLTGLGTGSGRTIGLRADMDALPMPEANDFDHASRHAGKMHACGHDGHTAMLLGAARYLA ETRNFDGTVHFIFQPAEEGLGGAKRMIDDGLFQQFDCEQVYGLHNWPELPAGRIAVHPGP VMAAANQFEIHVTGHGAHAAMPHRGIDPVLVSAHIVTAAQSLVSRGTNPAESAVVSITVV EAGTAANVIPDSARMRGTMRTFSEENHSRIQEQFGRLVTSIAEGLGATAELRFRPGYPAT VNSEPEARIAAAAAAKVVGEENVVWAPDPTMAAEDFGYMLKERPGAYVWLGHGGHLGSSC RLHNPHYDFNDAILTTGASYWASLVETILPRSA