Protein Info for MPMX19_02299 in Azospirillum sp. SherDot2

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 46 to 1104 (1059 residues), 1504.3 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 172 to 378 (207 residues), 226.4 bits, E=1.1e-70
  • amino acids 726 to 927 (202 residues), 124.6 bits, E=1.7e-39
• PF02787: CPSase_L_D3
  • amino acids 471 to 548 (78 residues), 87 bits, 2.7e-28
• PF02222: ATP-grasp
  • amino acids 733 to 898 (166 residues), 34.2 bits, E=8.3e-12
• PF02142: MGS
  • amino acids 1011 to 1093 (83 residues), 80 bits, 4.7e-26

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (3/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1126 amino acids)

>MPMX19_02299 Carbamoyl-phosphate synthase large chain (Azospirillum sp. SherDot2)
MPPLPPRPGTPYKGALHDMPIPLSVSGPPVPGGFRLTTFQRSGPMPKRTDIKSICIIGAG
PIVIGQACEFDYSGVQACKALREEGYRVILVNSNPATIMTDPGLADATYIEPITPAVVAK
ILEKERPDALLPTMGGQTALNTAMALSDDGTLERLGVEMIGAKRDVIAKAEDRILFRDAM
DKLGLESPKSRMVRTLDEAMDALELVGLPAIIRPSFTLAGTGGGIAYNRAEFEDIVRGGL
RASPVGEVLIEESVLGWKEYEMEVVRDGADNCIIVCAIENIDPMGVHTGDSITVAPSLTL
TDKEYQIMRDASIAVLREIGVDTGGSNVQFAVNPANGRLIVIEMNPRVSRSSALASKATG
FPIAKIAAKLAVGYRLDELTNDITGTTPASFEPTIDYVVTKMPRFTFEKFKGSEPLLTTS
MKSVGEAMSIGRTFQESVQKALRSMETGLTGFNEVKIGGDENPDRTAIRGALATPTPDRL
LVIAQAFRHGFTVDEVQAVSKYDPWFLEQIKAITDREAAIRADGLPTDKAGWLKLKQMGF
SDARLAELAKTTEAAVAAARRMAGVTPVYKRIDTCAAEFASATPYMYSTYETDGTGEAEC
ESDPTDKRKVVILGGGPNRIGQGIEFDYCCVHAVYALQEAGIETIMVNCNPETVSTDYDT
ADRLYFEPLTAEDVVELVRVEQRKGTVLGCIVQFGGQTPLKLAAALEAAGIPILGTSPDA
IDLAEDRERFQKLLHELNLKQPANGLARSLEEAEAVASRIGFPVVIRPSYVLGGRAMEIV
HDMAGLQRYMGTAVQVSGKNPVLIDSYLQDAIEVDVDVVCDGTDVYVAGVMEHIEEAGIH
SGDSACALPPYTLPADTIAEINRQSDALAHALKVVGLMNVQFAVKAGTVYILEVNPRASR
TVPFVAKATGTAIAKIAARIMAGEKLSAFTLNGPTPPHTAVKEAVFPFARFPGVDIVLGP
EMKSTGEVMGLDHNFALAFAKAQLGAGVTLPVKGSVFVSVKDHDKPALAVISKKLHEMGF
RILATSGTAKVLSDAGVPAETINKVVEGQPHIVDAMINGNVHLVINTTEGAQALSDSFSL
RRTALTYNIPYYTTVAGARAAVEAIAALRNGSLEVAPLQSYLSGSY