Protein Info for MPMX19_02294 in Azospirillum sp. SherDot2

Annotation: Periplasmic nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 833 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 29 (27 residues), 17.3 bits, see alignment (E = 4.8e-07) TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 830 (828 residues), 1577.3 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 41 to 93 (53 residues), 65.9 bits, see alignment 3.7e-22 PF00384: Molybdopterin" amino acids 97 to 570 (474 residues), 239.7 bits, see alignment E=7.6e-75 PF01568: Molydop_binding" amino acids 716 to 824 (109 residues), 90.1 bits, see alignment E=1.5e-29

Best Hits

Swiss-Prot: 77% identical to NAPA_BRASB: Periplasmic nitrate reductase (napA) from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 97% identity to azl:AZL_002680)

MetaCyc: 70% identical to periplasmic nitrate reductase subunit NapA (Escherichia coli K-12 substr. MG1655)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (833 amino acids)

>MPMX19_02294 Periplasmic nitrate reductase (Azospirillum sp. SherDot2)
MFLSRRDYLKAQAAAAAAAAAGISLPASAANILTGEGAKLTWSKAPCRFCGTGCGVMVGV
KDGRVVATHGDVKAEVNRGLNCVKGYFLSKIMYGEDRLNQPLLRMKDGKFDKDGEFEPIS
WDAAFDIMAAKWKETLKKKGPTAVGMFGSGQWTIYEGYAASKLMKAGLRSNNLDPNARHC
MASAVAGFMRTFGMDEPMGCYDDMEQADAFVLWGSNMAEMHPILWTRVTDRRLSHPGCKV
AVLSTYEHRSFDLADIGLVFTPGSDLAILNYIANHIIKTGRVNKEFIEKHCNFKRGRDDI
GYGLRPDNPLEKKAKNADKANDSDPISFDEFAKFVEPYTLDKAHELSGVPKNRLEALAEL
YADPKVKVVSFWTMGFNQHVRGVWANNLCYNIHLLTGKISQPGCGPFSLTGQPSACGTAR
EVGTFSHRLPADMVVTNPEHRRHAEEIWKLPEGTIPDKVGYHAVQQDRMLKDGKLNAYWV
MCNNNMQTAPNTAKETFPGYRNPENFIVVSDPYPTVTALAADLILPTAMWVEKEGGYGNA
ERRTQLWRQLVDAPDGARSDLWQIMEFSKRFRIEEVWPEELLNKKPELRGKTLYEVLFRN
GQVDRFPLSDLDQNYNNQEAKDAGFYVQKGLFEEYASFGRGHGHDLAPFEQYHQERGLRW
PVVDGKETKWRYREGSDPYVKAGEGMRFYGNKDGKANVFALPYEPPAEAPDTDYPFWLST
GRVIEHWHSGSMTLRVPELRKAFPNAVVFMHPDDAKDLNVRRGQEVRVTSRRGEVRVRVE
TRGRNKPPRGLVFVPFFDHTVLINKVTLDATDPISKQTDYKKCAVKITPVVNA