Protein Info for MPMX19_02229 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1246 transmembrane" amino acids 539 to 549 (11 residues), see Phobius details PF08471: Ribonuc_red_2_N" amino acids 31 to 147 (117 residues), 146.1 bits, see alignment E=3.5e-47 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 132 to 747 (616 residues), 475.3 bits, see alignment E=1.5e-146 PF02867: Ribonuc_red_lgC" amino acids 198 to 744 (547 residues), 350.3 bits, see alignment E=1.9e-108 amino acids 823 to 905 (83 residues), 55.8 bits, see alignment 3.3e-19

Best Hits

Swiss-Prot: 69% identical to NRDJ_BRADU: Vitamin B12-dependent ribonucleotide reductase (nrdJ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 98% identity to azl:AZL_002950)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1246 amino acids)

>MPMX19_02229 hypothetical protein (Azospirillum sp. SherDot2)
MRIERRFTHEGQDAYASLGFRQATSEIRNPDGTIVFQQTGIEVPDNFSQVASDILAQKYF
RKAGVPAALRAVEENTVPSWLWRKEADQEKLAALPKEKRFVGEHSAKQVFDRLAGTWTYW
GWKGGYFDTEADAHTFFDEMRFMLAAQMAAPNSPQWFNTGLHWAYGIDGPSQGHFYVDFK
TGLLTSSASAYEHPQPHACFIQSVADDLVNEGGIMDLWVREARLFKYGSGTGSNFSRLRG
EGEKLAGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVTVDMDHPDIEGYIDWKVNE
EQKVAALVTGSRICQKHLTSVMAAAQSGLDPKENKELKKAILAARKDQVSENYIQRVIQF
AGQGFTSIEFKTYNTDWDSEAYLTVSGQNSNNSVRISNEFVQAVLDDADWNLIGRTNNKV
MKTVRARDLWDKVGYAAWACADPGVQFDSTINEWHTCPASGRINASNPCSEYMFLDDTAC
NLASLNLMSFRLKDGSFDVEAFEHACRLWTIVLEISVLMAQFPSKEIAQLSYEFRTLGLG
FANLGGLLMASGLSYDSDEGRAYCAGISAVMTGVAYATSAEMAQELGTFPGFEANRDHML
RVIRNHRRAANGEMNGYEGLAVEPVPFVADLCPDQELALAAVRVWDEALELGEAHGYRNA
QATVVAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIVNRLVPEALKTLGYTPDQ
IEQIALYAVGHGTLKNAPGVNHEALKAKGFTDELLERLEGNLATAFDIKFVFNRWTIGAD
FCTQTLGIPAETLDAPGFDLLSHIGFSKSQIELANTFCCGAMTLEGAPFLKDEHLSVFDC
ANPCGRIGKRFLSWESHITMMAAAQPFISGAISKTINMPNTATVDECKDAYLMSWRLGLK
ANALYRDGSKLSQPLQAALLDDEENGEAVEAVIEAPAAVRAQMITERIVEKVIERVVERK
SSSRERLPHRRKGYTQKANVGGHKVYLRTGEYEDGRLGEIFIDMHKEGAAFRSLMNNFAI
AVSIGLQYGVPLEEFVEAFTFTRFEPSGMVTGNDTIKMATSIIDYIFREIAISYLNRTDL
AHATPEDLVPSTVGSGDKQGDLPDTQVQPSDIVRRIASTGYVRNNLRVLHGGQTQRASAA
AAFAATGTDTAAVSVTASAGTQVSAQAAMSASPAVAAATAQGHVAATAATGTAYGGSTND
QRFDRIREARARGYEGDPCGECGNMTLVRNGTCLKCDSCGSTTGCS