Protein Info for MPMX19_02117 in Azospirillum sp. SherDot2

Annotation: Bifunctional protein HldE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 TIGR02198: bifunctional protein RfaE, domain I" amino acids 9 to 316 (308 residues), 368.8 bits, see alignment E=3.2e-114 PF00294: PfkB" amino acids 15 to 307 (293 residues), 144.4 bits, see alignment E=7.4e-46 PF08543: Phos_pyr_kin" amino acids 195 to 293 (99 residues), 30.3 bits, see alignment E=4.3e-11 TIGR02199: bifunctional protein RfaE, domain II" amino acids 340 to 480 (141 residues), 208.3 bits, see alignment E=6.3e-66 TIGR00125: cytidyltransferase-like domain" amino acids 350 to 412 (63 residues), 51 bits, see alignment E=1.9e-17 PF01467: CTP_transf_like" amino acids 352 to 453 (102 residues), 59.6 bits, see alignment E=5.7e-20

Best Hits

Swiss-Prot: 62% identical to HLDE_MAGSA: Bifunctional protein HldE (hldE) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 88% identity to azl:AZL_025050)

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>MPMX19_02117 Bifunctional protein HldE (Azospirillum sp. SherDot2)
MSDLARHIDTLSRANVLCIGDVMLDRFVYGSVDRVSPEAPIPVLRVDREVPKLGGAGNVA
ANLVALEADCRFVSVVGRDGVGTDLLALLRREGVSDGAIVMEDGRQTTVKTRFIAGQQQL
LRTDIETVVPITVTDRVLERVRDLLPEVGGVILSDYGKGVLTDELVVAVIAAARQAGRTV
VVDPKGRDYRRYRGADIVTPNRKELMEATGLPARTDEEVIAAARHLIATCGIGAVVATRS
EQGMSVVTAEDAVHLPAEAREVFDVSGAGDTVVATLTAALSVGIDLVDSARLANLAAGIV
VGKVGTAVVRSAELLSGLHEQQWRHGEEKVLTRDAAAERAERWRLRGKRVGFTNGCFDLL
HPGHIALLKQARSACDVLVVGLNSDASVKRLKGESRPVQNETARATVLASLGCVDLVVIF
GEDTPETLIHALRPDVLVKGADYTIATVVGADFVQSYGGKVVLAELVQGQSTTNTIKRMK
G